Protein Info for DDA3937_RS10795 in Dickeya dadantii 3937

Annotation: serralysin family metalloprotease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 477 PF13688: Reprolysin_5" amino acids 65 to 231 (167 residues), 32.5 bits, see alignment E=2.9e-11 PF00413: Peptidase_M10" amino acids 99 to 200 (102 residues), 36 bits, see alignment E=2e-12 PF05572: Peptidase_M43" amino acids 148 to 220 (73 residues), 24.4 bits, see alignment E=7.7e-09 PF13583: Reprolysin_4" amino acids 155 to 231 (77 residues), 26.7 bits, see alignment E=1.3e-09 PF08548: Peptidase_M10_C" amino acids 258 to 477 (220 residues), 306 bits, see alignment E=3.8e-95 PF00353: HemolysinCabind" amino acids 342 to 368 (27 residues), 22.2 bits, see alignment (E = 3.4e-08) amino acids 351 to 385 (35 residues), 38.7 bits, see alignment 2.3e-13

Best Hits

Swiss-Prot: 98% identical to PRTA_DICCH: Serralysin A (prtA) from Dickeya chrysanthemi

KEGG orthology group: None (inferred from 100% identity to ddd:Dda3937_03861)

Predicted SEED Role

"Secreted alkaline metalloproteinase (EC 3.4.24.-), PrtA/B/C/G homolog" in subsystem Protein secretion by ABC-type exporters (EC 3.4.24.-)

Isozymes

Compare fitness of predicted isozymes for: 3.4.24.-

Use Curated BLAST to search for 3.4.24.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E0SC28 at UniProt or InterPro

Protein Sequence (477 amino acids)

>DDA3937_RS10795 serralysin family metalloprotease (Dickeya dadantii 3937)
MEKNLSSRDDDALHSLSASSSYDSVYDLLHYHERGNGLTINGKPSYSIEDAGEQITRDNV
SWNGSNVFGKSANLTFKFLQSARSTPDGDTGFVKFNAAQVAQAKLALQSWADLANITFTE
VTGNQSANVTFGNYTRDSSGRLDYGTQAYAYLPGSGSASGTTWYNYNVDNIRSPDKMEYG
RQTLTHEIGHALGLNHPGDYNAGEGNPTYSDVTYAEDTRQFSIMSYWSEKNTGGDFKGHY
AAGPMLDDIDAIQRLYGANMTTRTGDTVYGFNSNTDRDFYTATSSSKALIFSVWDAGGND
TFDFSGYSNNQRINLNEGSLSDVGGLKGNVSIAHGVTIENAIGGSGNDLLIGNNADNILR
GGAGDDILYGGGGADRLYGGSGRDTFVYTAVSDSKVAAPDWILDFQTGVDKIDLSALNTG
NNLHFVNQFSGSGGEILLNWDSSASVSNLYLNLDNNTSPEFLVKIVGQVSQTADFVV