Protein Info for DDA3937_RS06320 in Dickeya dadantii 3937

Annotation: glutamine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 552 PF00749: tRNA-synt_1c" amino acids 28 to 337 (310 residues), 413.5 bits, see alignment E=7.1e-128 TIGR00440: glutamine--tRNA ligase" amino acids 28 to 551 (524 residues), 975.8 bits, see alignment E=3.1e-298 PF03950: tRNA-synt_1c_C" amino acids 340 to 440 (101 residues), 113.2 bits, see alignment E=8.2e-37 PF20974: tRNA-synt_1c_C2" amino acids 457 to 529 (73 residues), 88.3 bits, see alignment E=7.2e-29

Best Hits

Swiss-Prot: 92% identical to SYQ_PECCP: Glutamine--tRNA ligase (glnS) from Pectobacterium carotovorum subsp. carotovorum (strain PC1)

KEGG orthology group: K01886, glutaminyl-tRNA synthetase [EC: 6.1.1.18] (inferred from 100% identity to ddd:Dda3937_01562)

MetaCyc: 85% identical to glutamine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Glutamine--tRNA ligase. [EC: 6.1.1.18]

Predicted SEED Role

"Glutaminyl-tRNA synthetase (EC 6.1.1.18)" in subsystem tRNA aminoacylation, Glu and Gln (EC 6.1.1.18)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E0SEW5 at UniProt or InterPro

Protein Sequence (552 amino acids)

>DDA3937_RS06320 glutamine--tRNA ligase (Dickeya dadantii 3937)
MSEAEARPTNFIRQIIDEDLATGKHDHIQTRFPPEPNGYLHIGHAKSICLNFGIAEDYNG
KCNLRFDDTNPVKEDIEYVESIKHDVQWLGFSWSGNVRYSSDYFDQLHFYAIELINKGLA
YVDELTPEQIREYRGTLTSPGKNSPFRDRSVEENLTLFEKMRSGGFAEGTACLRAKIDMA
SPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCISDALEGITHSLCTLEFQDNRRL
YDWVLDNISIPAHPRQYEFSRLNLEYAVMSKRKLNQLVSENVVEGWSDPRMPTISGLRRR
GYTAASIREFCRRIGVTKQDNTVEMAALESCIRDDLNENAPRAMAVLDPVKVVLENLPAG
HEEWLAMPNHPNKPEMGSRQVAFSREVYIDRADFREEANKQYKRLVLGKEVRLRNAYVIK
AERIDKDEQGVITTIYCSCDTETLSKDPADGRKVKGVIHWVSAAHALPAQFRLYDRLFSV
ANPGAAEDFLSTINPDSLTVAQGFVEASLGQAEVEKAYQFEREGYFCADRVYSSADNLVF
NRTVGLRDSWAG