Protein Info for CA264_12265 in Pontibacter actiniarum KMM 6156, DSM 19842

Annotation: DNA primase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 639 TIGR01391: DNA primase" amino acids 4 to 414 (411 residues), 467.4 bits, see alignment E=2e-144 PF01807: zf-CHC2" amino acids 5 to 100 (96 residues), 125.7 bits, see alignment E=1.9e-40 PF08275: DNAG_N" amino acids 126 to 255 (130 residues), 140.7 bits, see alignment E=1e-44 PF13362: Toprim_3" amino acids 265 to 354 (90 residues), 28.6 bits, see alignment E=5.7e-10 PF13155: Toprim_2" amino acids 265 to 350 (86 residues), 80.4 bits, see alignment E=3.6e-26 PF01751: Toprim" amino acids 266 to 329 (64 residues), 32.9 bits, see alignment E=2.2e-11 PF13662: Toprim_4" amino acids 266 to 338 (73 residues), 53.9 bits, see alignment E=6.1e-18 PF10410: DnaB_bind" amino acids 384 to 424 (41 residues), 22.4 bits, see alignment 4.2e-08

Best Hits

Predicted SEED Role

"DNA primase (EC 2.7.7.-)" in subsystem DNA-replication or Macromolecular synthesis operon (EC 2.7.7.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.-

Use Curated BLAST to search for 2.7.7.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1X9YTJ6 at UniProt or InterPro

Protein Sequence (639 amino acids)

>CA264_12265 DNA primase (Pontibacter actiniarum KMM 6156, DSM 19842)
MSLIPKEVVDQVLAQADITEVVGDFVSLKKKGQNMWACCPFHHEKSPSFSVSPAKGIYKC
FGCGKAGNSVQFIMDVEGTSFPEAIKYLAKKYSIEVPEEQHDPEYAREQSERDSLFIVSD
FAAKHFQQRLTSHEQGAIGQSYLKQRGLSSNTIRKFELGYSLDEWTDLTDAALKVGYKQK
YLEATGLTIAREDGKKYDRFRGRVMFPIHNVSGRVVGFGARTLKSNDKKSPKYLNSPESD
IYHKSNVLYGLFQAKQAMRMQDMCYMVEGYLDVLSLHQGGIENVVASSGTSLTEGQIKLI
SRYTENITVLYDGDAAGIKASLRGIDLILELGLNVNVVTFPAGEDPDSYIHKVGDTAFKT
YIKEHSQDFISFKTSLFAEEAAGNPVKKAEAIKEVVASISKIPDAIKRTVFFQSCSLIFD
IDEQVLISEYNKMHLQGRQQQPSGGQAQHYSAPEPALEPEPEKPASDTDVLKRYEREIIR
MMINYGAKPVDEQQTVTQYMLEQLEDVEFEIPIYVKLYNLILRQFNQGYVVKGEDLVNDP
DEEIRSEVIELISNRYDLSHNWETHQIYVPNEEDLLMYGIERAILRLKRMKVQILLKAEL
EKLRLVTDPAEQDRQLNYILSLKTFENQLGEMLGIVVNK