Protein Info for BNILDI_17070 in Escherichia coli ECRC62

Name: ynfE
Annotation: Putative dimethyl sulfoxide reductase chain YnfE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 808 signal peptide" amino acids 1 to 41 (41 residues), see Phobius details TIGR02166: anaerobic dimethyl sulfoxide reductase, A subunit, DmsA/YnfE family" amino acids 10 to 808 (799 residues), 1395.4 bits, see alignment E=0 PF04879: Molybdop_Fe4S4" amino acids 49 to 108 (60 residues), 49.6 bits, see alignment 4.7e-17 PF00384: Molybdopterin" amino acids 111 to 566 (456 residues), 233.9 bits, see alignment E=4.4e-73 PF01568: Molydop_binding" amino acids 687 to 800 (114 residues), 93.6 bits, see alignment E=1.2e-30

Best Hits

Swiss-Prot: 99% identical to YNFE_ECOLI: Putative dimethyl sulfoxide reductase chain YnfE (ynfE) from Escherichia coli (strain K12)

KEGG orthology group: K07309, Tat-targeted selenate reductase subunit YnfE [EC: 1.97.1.9] (inferred from 100% identity to eck:EC55989_1752)

MetaCyc: 70% identical to putative selenate reductase YnfF (Escherichia coli K-12 substr. MG1655)
Selenate reductase. [EC: 1.97.1.9]

Predicted SEED Role

No annotation

Isozymes

Compare fitness of predicted isozymes for: 1.97.1.9

Use Curated BLAST to search for 1.97.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (808 amino acids)

>BNILDI_17070 Putative dimethyl sulfoxide reductase chain YnfE (Escherichia coli ECRC62)
MSKNDRMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAATVQQASEKVIWGACSVNC
GSRCALRLHVKDNEVTWVETDNTGSDEYGNHQVRACLRGRSIRRRINHPDRLNYPMKRVG
TRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYSSGIVGGNMTRSSPSASAVKR
LMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTDIENSKLVVMFGNNPAETRMSGG
GITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWLPIRPGTDAALVAGIAWVLINENLV
DQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGEGDDNTAKTPQWASQITGIPVDRIIKL
AREIGTAKPAYICQGWGPQRQANGELTARAIAMLPILTGNVGISGGNSGARESTYTITIE
RLPVLDNPVKTSISCFSWTDAIDHGPQMTAIRDGVRGKDKLDVPIKFIWNYAGNTLVNQH
SDINKTHEILQDESKCEMIVVIENFMTSSAKYADILLPDLMTVEQEDIIPNDYAGNMGYL
IFLQPVTSEKFERKPIYWILSEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALP
SYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEK
DEVISPLPVYASTFEGWDSPERRTFPLQLFGFHYKSRTHSTYGNIDLLKAACRQEVWINP
IDAQKRGIANGDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGC
VNTLTTLRPSPLAKGNPQHTNLVEIEKI