Protein Info for BNILDI_04250 in Escherichia coli ECRC62

Name: yidJ
Annotation: Uncharacterized sulfatase YidJ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 497 PF00884: Sulfatase" amino acids 4 to 341 (338 residues), 243.8 bits, see alignment E=2.7e-76 PF16347: SGSH_C" amino acids 300 to 434 (135 residues), 61.2 bits, see alignment E=1.4e-20

Best Hits

Swiss-Prot: 99% identical to YIDJ_ECOLI: Uncharacterized sulfatase YidJ (yidJ) from Escherichia coli (strain K12)

KEGG orthology group: K01138, uncharacterized sulfatase [EC: 3.1.6.-] (inferred from 99% identity to eco:b3678)

Predicted SEED Role

"Choline-sulfatase (EC 3.1.6.6)" in subsystem Choline Transport or Choline and Betaine Uptake and Betaine Biosynthesis (EC 3.1.6.6)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 3.1.6.-

Use Curated BLAST to search for 3.1.6.- or 3.1.6.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (497 amino acids)

>BNILDI_04250 Uncharacterized sulfatase YidJ (Escherichia coli ECRC62)
MKRPNFLFIMTDTQATNMVGCYSGKQLNTQNIDSLAAEGIRFNSAYTCSPVCTPARAGLF
TGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWHLDGHDYFGTGECPPEWDA
DYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETFTWAHRISNRAVDFLQQPARA
EEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYDLGEKAQDDLANKPEHHRLWAQAMPSPV
GDDGLYHHPLYFACNDFVDDQIGRVINALTPEQRENTWVIYTSDHGEMMGAHKLISKGAA
MYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPTMMALADIEKPEILPGENILAVKEPRG
VMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLVLNLFTSDELYDRRNDPNEMHNLIDDIRF
ADVRSKMHDALLDYMDKIRDPFRSYQWSLRPWRKDALPRWMGAFRPRPQDGYSPVVRDYD
TGLPTQGVKVEEKKQKF