Protein Info for BACOVA_03732 in Bacteroides ovatus ATCC 8483

Annotation: UDP-N-acetylglucosamine 4-epimerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 330 signal peptide" amino acids 1 to 17 (17 residues), see Phobius details transmembrane" amino acids 250 to 269 (20 residues), see Phobius details PF04321: RmlD_sub_bind" amino acids 1 to 186 (186 residues), 43 bits, see alignment E=1.1e-14 PF01370: Epimerase" amino acids 3 to 219 (217 residues), 104.5 bits, see alignment E=2.1e-33 PF01073: 3Beta_HSD" amino acids 5 to 240 (236 residues), 79.3 bits, see alignment E=8.8e-26 PF13460: NAD_binding_10" amino acids 7 to 166 (160 residues), 49 bits, see alignment E=2.5e-16 PF16363: GDP_Man_Dehyd" amino acids 7 to 215 (209 residues), 44.8 bits, see alignment E=4.2e-15 PF02719: Polysacc_synt_2" amino acids 32 to 190 (159 residues), 24 bits, see alignment E=7.5e-09 PF07993: NAD_binding_4" amino acids 76 to 192 (117 residues), 33.8 bits, see alignment E=7.3e-12

Best Hits

Swiss-Prot: 66% identical to GNU_ECO57: N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol 4-epimerase (gnu) from Escherichia coli O157:H7

KEGG orthology group: None (inferred from 71% identity to bfr:BF3647)

MetaCyc: 66% identical to N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol 4-epimerase (Escherichia coli O157)
RXN-14572 [EC: 5.1.3.26]

Predicted SEED Role

"UDP-glucose 4-epimerase (EC 5.1.3.2)" in subsystem Lacto-N-Biose I and Galacto-N-Biose Metabolic Pathway or Lactose and Galactose Uptake and Utilization or N-linked Glycosylation in Bacteria or Rhamnose containing glycans or linker unit-arabinogalactan synthesis (EC 5.1.3.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 5.1.3.2

Use Curated BLAST to search for 5.1.3.2 or 5.1.3.26

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (330 amino acids)

>BACOVA_03732 UDP-N-acetylglucosamine 4-epimerase (Bacteroides ovatus ATCC 8483)
MKITLIGASGFVGTRLITLLKKAQRYEMLNIDLQQSRFFPEVTAIGDVRDQDYLDKKLEG
AECVVLLAAQHRDDVVPVSLYYDTNVGGIEKTLKAMEKNNIKRIIFFSSVAVYGLNKKNP
NEEYPADPFNHYGKSKWQAEQILHEWYKLHPDWNIDIIRPTVIFGERNRGNVYNLLKQIA
SGKFLMIGSGNNKKSMAYVGNVVAFVKYMIDEVRTGYNVFNYVDKPDFTMNELVAHVSKV
MNKHIPATHFPYWLGMIGGYCFDIFACITRKKLAVSSVRVKKFCATTEFDAAKIHSSVFK
APYSLGEGLARTLEFEFIYPPTDDITFKSE