Protein Info for BACOVA_02895 in Bacteroides ovatus ATCC 8483

Annotation: tRNA-i(6)A37 thiotransferase enzyme MiaB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 457 TIGR00089: radical SAM methylthiotransferase, MiaB/RimO family" amino acids 21 to 454 (434 residues), 458.8 bits, see alignment E=1.7e-141 TIGR01574: tRNA-i(6)A37 thiotransferase enzyme MiaB" amino acids 21 to 455 (435 residues), 505.4 bits, see alignment E=1.5e-155 PF00919: UPF0004" amino acids 21 to 119 (99 residues), 100.8 bits, see alignment E=7.1e-33 PF04055: Radical_SAM" amino acids 167 to 335 (169 residues), 99.3 bits, see alignment E=5.8e-32 PF01938: TRAM" amino acids 395 to 455 (61 residues), 56.4 bits, see alignment E=4.3e-19 PF18693: TRAM_2" amino acids 398 to 456 (59 residues), 27.4 bits, see alignment E=6.2e-10

Best Hits

Swiss-Prot: 97% identical to MIAB_BACTN: tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase (miaB) from Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)

KEGG orthology group: K06168, bifunctional enzyme involved in thiolation and methylation of tRNA (inferred from 97% identity to bth:BT_3195)

Predicted SEED Role

"tRNA-i(6)A37 methylthiotransferase" in subsystem Ribosomal protein S12p Asp methylthiotransferase or tRNA processing

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (457 amino acids)

>BACOVA_02895 tRNA-i(6)A37 thiotransferase enzyme MiaB (Bacteroides ovatus ATCC 8483)
MNELTGADFKSATEMTDDNKKLFIETYGCQMNVADSEVIASVMQMAGYSVAETLEEADAV
FMNTCSIRDNAEQKILNRLEFFHSLKKKKKRLIVGVLGCMAERVKDDLITNHHVDLVVGP
DAYLTLPDLIAAVETGEKAINVELSTTETYRDVIPSRICGNHISGFVSIMRGCNNFCTYC
IVPYTRGRERSRDVESILNEVADLVAKGYKEVTLLGQNVNSYRFERPTGEVVTFPMLLRT
VAEAAPGVRIRFTTSHPKDMSDETLEVIAQVPNVCKHIHLPVQSGSSRILKLMNRKYTRE
WYLDRVAAIKRIIPDCGLTTDIFSGFHSETEEDHAMSLSLMEACGYDAAFMFKYSERPGT
YASKHLEDNVPEEVKVRRLNEIIALQNRLSAESNQRCIGKTYEVLVEGVSKRSRDQLFGR
TEQNRVVVFDRGTHRVGDFVNVRVTEASSATLKGEEI