Protein Info for BACOVA_00830 in Bacteroides ovatus ATCC 8483
Annotation: nitrite reductase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 97% identical to NRFA_BACTN: Cytochrome c-552 (nrfA) from Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
KEGG orthology group: K03385, formate-dependent nitrite reductase, periplasmic cytochrome c552 subunit [EC: 1.7.2.2] (inferred from 97% identity to bth:BT_1417)MetaCyc: 47% identical to cytochrome c552 nitrite reductase (Escherichia coli K-12 substr. MG1655)
Nitrite reductase (cytochrome; ammonia-forming). [EC: 1.7.2.2]
Predicted SEED Role
"Cytochrome c552 precursor (EC 1.7.2.2)" in subsystem Nitrate and nitrite ammonification or Soluble cytochromes and functionally related electron carriers (EC 1.7.2.2)
MetaCyc Pathways
- nitrate reduction IV (dissimilatory) (1/2 steps found)
- formate to nitrite electron transfer (1/3 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.7.2.2
Use Curated BLAST to search for 1.7.2.2
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (493 amino acids)
>BACOVA_00830 nitrite reductase (Bacteroides ovatus ATCC 8483) MEKKLKSWQGWLLFGGSMVVVFVLGLCVSALMERRAEVASIFNNRKNVIKGIEARNELFK NDFPREYQTWTETAKTDFESEFNGNIAVDALEKRPEMVILWAGYAFSKDYSTPRGHMHAI EDITASLRTGSPMSPTEGPQPSTCWTCKSPDVPRMMEALGVDSFYNNKWGAMGAEIVNPI GCSDCHDPETMNLHISRPALIEAFQRQGKDITKATPQEMRSLVCAQCHVEYYFKGDGKYL TFPWDKGFTVEDMEAYYDEAGFYDYIHKLSRTPILKAQHPDYEICQMGIHGQRGVSCADC HMPYKSEGGVKFSDHHIQSPLAMIDRTCQVCHRESEETLRNNVYERQRKANEIRNRLEQE LAKAHIEAKFAWDKGATETQMKDVLALIRQAQWRWDFGVASHGGSFHAPQEIQRILSHGL DRAMQARLAVSKVLAKNGYTGDVPMPDISTKAKAQEYIGLDMDAERAAKEKFLKTTVPAW LEKAKENGRLAQK