Protein Info for B158DRAFT_2529 in Kangiella aquimarina DSM 16071

Annotation: NAD synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00795: CN_hydrolase
  • amino acids 2 to 239 (238 residues), 95.3 bits, E=5.8e-31
• TIGR00552: NAD+ synthetase
  • amino acids 258 to 514 (257 residues), 205.8 bits, E=3.1e-65
• PF02540: NAD_synthase
  • amino acids 261 to 510 (250 residues), 250.5 bits, E=2.1e-78

Best Hits

KEGG orthology group: K01950, NAD+ synthase (glutamine-hydrolysing) [EC: 6.3.5.1] (inferred from 92% identity to kko:Kkor_1079)

Predicted SEED Role

"NAD synthetase (EC 6.3.1.5) / Glutamine amidotransferase chain of NAD synthetase" in subsystem NAD and NADP cofactor biosynthesis global or NAD regulation (EC 6.3.1.5)

MetaCyc Pathways

• NAD de novo biosynthesis II (from tryptophan) (9/9 steps found)
• aspartate superpathway (20/25 steps found)
• NAD de novo biosynthesis I (6/6 steps found)
• NAD de novo biosynthesis IV (anaerobic) (6/6 steps found)
• NAD biosynthesis from 2-amino-3-carboxymuconate semialdehyde (4/4 steps found)
• NAD de novo biosynthesis III (5/6 steps found)
• NAD salvage pathway II (PNC IV cycle) (4/5 steps found)
• NAD salvage pathway I (PNC VI cycle) (4/7 steps found)
• superpathway of NAD biosynthesis in eukaryotes (9/14 steps found)
• NAD salvage pathway V (PNC V cycle) (2/5 steps found)
• NAD salvage (plants) (4/11 steps found)

KEGG Metabolic Maps

• Glutamate metabolism
• Nicotinate and nicotinamide metabolism
• Nitrogen metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.1.5 or 6.3.5.1

Protein Sequence (540 amino acids)

>B158DRAFT_2529 NAD synthase (Kangiella aquimarina DSM 16071)
MRIAIAQNNYTVGDIRHNLELIKSSLKQAESDNADIVVFSELSLCGYPPEDLLLRPDLYT
IIDDAIDELLSLNSNVAMVIGHPIKEPEGLFNVATVIHQNKILVQYRKQLLPNYSVFDEK
RYFESGSEDCVFEFKGVNVGLLVCEDLWYPEPTARVKALGADILLSPNASPYCYGKGALR
SAEMGARARENELPIVYVNQIGGQDELIFDGCSAVYNEEGQRVFQMAEMAEDFAFIDFDK
TSGAIKPSKGYLNQFHGEEEIWQALVLGVRDYAHKNGFKGALLGLSGGIDSAVTLAVAVD
ALGKENVNAVMMPFRYTASMSIEDAEAEAKALGIEFDVISIEPMYEAFMSQLSSQFAGTE
TDLTEQNIQARCRGTLLMALSNKFGRLVLTTGNKSEVAVGYCTLYGDMAGGLDVLKDVSK
TMVYRLARYRNTISPVIPERVITRPPSAELAPDQKDEDNLPPYDILDAILEAYVEQDKGI
DDIVAMGYDEADVRRVIWLVDINEHKRRQAPPGIRITQRAFGRDRRYPITSGFGRQFKSK