Protein Info for B158DRAFT_2268 in Kangiella aquimarina DSM 16071

Annotation: single-stranded-DNA-specific exonuclease RecJ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 575 transmembrane" amino acids 185 to 202 (18 residues), see Phobius details TIGR00644: single-stranded-DNA-specific exonuclease RecJ" amino acids 20 to 569 (550 residues), 524 bits, see alignment E=1.8e-161 PF01368: DHH" amino acids 70 to 230 (161 residues), 84.5 bits, see alignment E=1.2e-27 PF02272: DHHA1" amino acids 319 to 450 (132 residues), 77 bits, see alignment E=3e-25 PF17768: RecJ_OB" amino acids 465 to 569 (105 residues), 80.4 bits, see alignment E=1.5e-26

Best Hits

Swiss-Prot: 50% identical to RECJ_ECOLI: Single-stranded-DNA-specific exonuclease RecJ (recJ) from Escherichia coli (strain K12)

KEGG orthology group: K07462, single-stranded-DNA-specific exonuclease [EC: 3.1.-.-] (inferred from 83% identity to kko:Kkor_0835)

MetaCyc: 50% identical to ssDNA-specific exonuclease RecJ (Escherichia coli K-12 substr. MG1655)
Exodeoxyribonuclease VII. [EC: 3.1.11.6]

Predicted SEED Role

"Single-stranded-DNA-specific exonuclease RecJ (EC 3.1.-.-)" in subsystem DNA-replication or DNA Repair Base Excision or DNA repair, bacterial RecFOR pathway (EC 3.1.-.-)

Isozymes

Compare fitness of predicted isozymes for: 3.1.-.-, 3.1.11.6

Use Curated BLAST to search for 3.1.-.- or 3.1.11.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (575 amino acids)

>B158DRAFT_2268 single-stranded-DNA-specific exonuclease RecJ (Kangiella aquimarina DSM 16071)
MQKLIRQRQLNQEVQPLTASPVLNRIYASRGVEDPDELDYSLTKLLPYHQLKGIEAAVNL
LFEAIHQQRRILIVGDFDADGATSSALTKLVLTSFGAKHVDFLVPNRFEFGYGLSPELVE
VAKESRPDLIITVDNGISSIAGVKAAREHGIKVLVTDHHLAGEELPEADAIVNPNQPGDA
FPSKNLAGVGVVFYVLLALRARLREENWFESQSLEEPNLAHYLDIVALGTVADVVPLDAN
NRLLVHNGLRLIRAGRSRPGINALLQLAKRQLAKVTAMDLGFAVGPRLNAAGRLDDMAIG
IECLLAEDKAQATELAEILHSLNSDRQQVEQEMQDEALSKLDDIDWQSGEFAHSICLYEP
HWHQGVVGLLASRIKERVNKPCIVFANADDNSVKGSARSVKEVHIRDALALVDSRHPQLI
EKFGGHAMAAGLSLALENLEPFQNAFEQAVTDLLKGKPIEDVIETDGELNPDELTLDTAM
ELSFAGPFGQGFPEPAFDGEFEVLNHRIVGENHLKLTLRSPEQDHALQAIAFRIDVEEWL
SQPASKIRAVYKPDINDYYNVPELQLLIQYFERLA