Protein Info for B158DRAFT_2244 in Kangiella aquimarina DSM 16071

Annotation: phosphoribosylformylglycinamidine synthase, single chain form

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1298 TIGR01735: phosphoribosylformylglycinamidine synthase" amino acids 3 to 1296 (1294 residues), 1975.3 bits, see alignment E=0 PF18076: FGAR-AT_N" amino acids 35 to 152 (118 residues), 134.4 bits, see alignment E=3.7e-43 PF18072: FGAR-AT_linker" amino acids 173 to 222 (50 residues), 78.8 bits, see alignment (E = 7.1e-26) PF02769: AIRS_C" amino acids 433 to 590 (158 residues), 116.9 bits, see alignment E=2e-37 amino acids 839 to 969 (131 residues), 58.4 bits, see alignment E=1.9e-19 PF13507: GATase_5" amino acids 1042 to 1296 (255 residues), 370.5 bits, see alignment E=7.2e-115

Best Hits

Swiss-Prot: 64% identical to PUR4_ALIF1: Phosphoribosylformylglycinamidine synthase (purL) from Aliivibrio fischeri (strain ATCC 700601 / ES114)

MetaCyc: 65% identical to phosphoribosylformylglycinamide synthetase (Escherichia coli K-12 substr. MG1655)
Phosphoribosylformylglycinamidine synthase. [EC: 6.3.5.3]

Predicted SEED Role

"Phosphoribosylformylglycinamidine synthase, synthetase subunit (EC 6.3.5.3) / Phosphoribosylformylglycinamidine synthase, glutamine amidotransferase subunit (EC 6.3.5.3)" in subsystem De Novo Purine Biosynthesis (EC 6.3.5.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.5.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1298 amino acids)

>B158DRAFT_2244 phosphoribosylformylglycinamidine synthase, single chain form (Kangiella aquimarina DSM 16071)
MLILRGNPAFSNFQKAHLLKESSAISSQVIDIYGEYVHYVDTNSELTDEELKILERLLEY
GPSRPASEHSGQRLIVVPRPGTISPWSSKATNIAQNCSLDKIERIERAVAVYFKTKDGAE
LDADVIEQLKPLIHDRMTQSVFTEHSQAEVLFEQHQPKPLAHVDILGGGREALVKANSEI
GLALAEDEIDYLVESFIKLGRNPSDVELMMFAQANSEHCRHKIFNASWTIDGIEKEMSLF
KMIKNTYQVNSEGVLSAYKDNAAVFEGFTAHRFFANAETHEYQYHLEPVHVLCKVETHNH
PTCISPYPGASTGSGGEIRDEGATGIGGKPKVGLTGFTVSNLKVPGYIQPWETDYGKPER
IVTALDIMLEGPIGGASFNNEFGRPAVNGYFRTFEEEFDFEYGKEVRGYHKPIMIAGGMG
NIREQHVEKKEIKPGYKVIVLGGPAMLIGLGGGAASSMASGSSQEDLDFASVQRDNPEME
RRCQEVIDRCWAMGEKNPIEFIHDVGAGGLSNAIPELVHDGERGGKFELRRVPNAEKGMA
PVEIWCNEAQERYVLAVSPDSLAKFEEICGRERCPFAVVGEATEELHLELNDQHFGNKPV
DLPMDILFGKPPKMHREAATLAAPSTALPQVDDINEAVDRILALPAVADKTFLITIGDRT
VGGMVSRDQMVGPWQVPVADCAVTTSTHNSYTGEAMAMGERTPAALLDSAASARMAVGEA
VTNIAAAKIDDIKKIRLSANWMAAAGAPGEDVNLYKAVEAVGMQLCPELGITIPVGKDSM
SMKTVWEDNGEKKSVTAPLSLIITAFAPVTDVRKSLTPQLRMDKEATELLLLDLGRGKNR
LGATAFTQVYKLLGTEPANVDSSDDLRNFFNAVQALNHNDLVHAYHDRSDGGLLTTLIEM
GFAGNCGVSVDLSELNGTAEEILFNEELGAVLQVPADKREQVEAILAEHKVNDFAHFIGE
PAETKTFSVTKDGNDVISRDMKDLRAIWSELTYRMQAMRDNPECAQQEFDTKLDLDNPGI
KPVATYNVDENIAKPLIASGARPRVAVLREQGVNSQLEMAAAFTKAGFECIDVHMSDILS
GRVSLQDFTGAVACGGFSYGDVLGAGEGWAKSILFNSRARDEFEAFFNRQDTFSLGICNG
CQMMSNLKELIPGADHWPHFVRNQSAQFEARFAMVEVAKSPSLFMTGMEGSRLPIAVSHG
EGRAEFKSEQLANQVNESGLVTARYINNYGNIADTYPANPNGSPFGITGLTTTDGRVTIM
MPHPERVARTVQNSWRPDEWREDGAWQRMFYNARKHIG