Protein Info for B158DRAFT_2228 in Kangiella aquimarina DSM 16071

Annotation: dihydroorotate oxidase A (EC 1.3.3.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01036: dihydroorotate dehydrogenase (fumarate)
  • amino acids 2 to 339 (338 residues), 446.5 bits, E=2.7e-138
• PF01180: DHO_dh
  • amino acids 46 to 337 (292 residues), 275.2 bits, E=3.2e-86

Best Hits

Swiss-Prot: 57% identical to PYRD_ALISL: Dihydroorotate dehydrogenase (quinone) (pyrD) from Aliivibrio salmonicida (strain LFI1238)

KEGG orthology group: K00226, dihydroorotate dehydrogenase (fumarate) [EC: 1.3.98.1] (inferred from 92% identity to kko:Kkor_0794)

MetaCyc: 62% identical to dihydroorotate dehydrogenase, type 2 (Escherichia coli K-12 substr. MG1655)
Dihydroorotate dehydrogenase. [EC: 1.3.5.2]; 1.3.5.2 [EC: 1.3.5.2]; 1.3.5.2 [EC: 1.3.5.2]

Predicted SEED Role

"Dihydroorotate dehydrogenase (EC 1.3.3.1)" in subsystem De Novo Pyrimidine Synthesis (EC 1.3.3.1)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (42/46 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• superpathway of pyrimidine ribonucleotides de novo biosynthesis (9/9 steps found)
• UMP biosynthesis I (6/6 steps found)
• UMP biosynthesis II (6/6 steps found)

KEGG Metabolic Maps

• Pyrimidine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.3.3.1 or 1.3.5.2 or 1.3.98.1

Protein Sequence (342 amino acids)

>B158DRAFT_2228 dihydroorotate oxidase A (EC 1.3.3.1) (Kangiella aquimarina DSM 16071)
MYSLLRSLLFMLDGERSHHLSLNSLNLLNRLKLSGLLSQTQIDDPVEVMGIKFPNRVGLA
AGLDKNGEYIDALSHFGFGFIEVGTVTPRPQPGNPKPRLFRIKDAEAIINRMGFNNDGVD
ALLNNVEKAQFTGVLGINIGKNFDTPVEKAVDDYLIGMEKIYRRADYMVVNISSPNTPGL
RDLQFGETLKSLLENLKKKQQELQQEHKVYKPLAIKIAPDLSATDIDQLADTFRQFELDG
IIATNTTFDRSKVEGLKYAEEQGGLSGKPLASQSTKVIKRLVEALDDSIPVIGVGGITDE
QSAIDKIEAGAKLVQIYSGFIYHGPQLVYEVAKALKEHQQSQ