Protein Info for B158DRAFT_2064 in Kangiella aquimarina DSM 16071

Annotation: [protein release factor]-glutamine N5-methyltransferase (EC 2.1.1.-)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 277 TIGR00536: methyltransferase, HemK family" amino acids 5 to 276 (272 residues), 262.9 bits, see alignment E=2.9e-82 PF17827: PrmC_N" amino acids 8 to 76 (69 residues), 76.8 bits, see alignment E=6.9e-25 TIGR03534: protein-(glutamine-N5) methyltransferase, release factor-specific" amino acids 26 to 273 (248 residues), 304.7 bits, see alignment E=4.9e-95 PF05175: MTS" amino acids 104 to 195 (92 residues), 70.2 bits, see alignment E=7.5e-23 PF06325: PrmA" amino acids 105 to 187 (83 residues), 42.1 bits, see alignment E=3.6e-14 PF10294: Methyltransf_16" amino acids 111 to 184 (74 residues), 23.5 bits, see alignment E=1.8e-08 PF13847: Methyltransf_31" amino acids 113 to 242 (130 residues), 61.5 bits, see alignment E=3.8e-20 PF13649: Methyltransf_25" amino acids 117 to 198 (82 residues), 40.8 bits, see alignment E=1.3e-13 PF08241: Methyltransf_11" amino acids 119 to 187 (69 residues), 24.9 bits, see alignment E=1.1e-08 PF01170: UPF0020" amino acids 129 to 190 (62 residues), 22.6 bits, see alignment E=3.6e-08

Best Hits

Swiss-Prot: 50% identical to PRMC_VIBCH: Release factor glutamine methyltransferase (prmC) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K02493, methyltransferase [EC: 2.1.1.-] (inferred from 75% identity to kko:Kkor_0632)

MetaCyc: 47% identical to protein-(glutamine-N5) methyltransferase (Escherichia coli K-12 substr. MG1655)
RXN-14992 [EC: 2.1.1.297]

Predicted SEED Role

"Protein-N(5)-glutamine methyltransferase PrmC, methylates polypeptide chain release factors RF1 and RF2"

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.-

Use Curated BLAST to search for 2.1.1.- or 2.1.1.297

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (277 amino acids)

>B158DRAFT_2064 [protein release factor]-glutamine N5-methyltransferase (EC 2.1.1.-) (Kangiella aquimarina DSM 16071)
MEAKSVAQALTWARQQLESMDEAAIDADILLSHVMDKSRTWLKTWPEHILSKQQFSHYQE
SIERRKKGEPTAYIIGEKDFWSLTLKVTRDTLIPRPETELLVELALQRIPETSDFKVADL
GTGSGAIALAIAKERPQARVWALDMSEGALTVAQENAEHNKIKNVALEQGSWLSNWQHGQ
LDMIVSNPPYVAPNDPHLADLVYEPVTALVAEDNGLSDIYTITQQAIQHLKPNGFLLFEH
GYDQGSAVREILQSAGFDQVETVKDYAGQDRVTLGKR