Protein Info for B158DRAFT_1122 in Kangiella aquimarina DSM 16071

Annotation: aspartyl-tRNA synthetase (EC 6.1.1.12)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 590 TIGR00459: aspartate--tRNA ligase" amino acids 5 to 587 (583 residues), 857.8 bits, see alignment E=1.9e-262 PF01336: tRNA_anti-codon" amino acids 22 to 106 (85 residues), 69.5 bits, see alignment E=3.9e-23 PF00152: tRNA-synt_2" amino acids 123 to 559 (437 residues), 435.5 bits, see alignment E=2e-134 PF02938: GAD" amino acids 315 to 410 (96 residues), 89.8 bits, see alignment E=2.2e-29

Best Hits

Swiss-Prot: 68% identical to SYDND_PSEA6: Aspartate--tRNA(Asp/Asn) ligase (aspS) from Pseudoalteromonas atlantica (strain T6c / ATCC BAA-1087)

KEGG orthology group: K01876, aspartyl-tRNA synthetase [EC: 6.1.1.12] (inferred from 96% identity to kko:Kkor_1866)

MetaCyc: 66% identical to aspartate--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Aspartate--tRNA ligase. [EC: 6.1.1.12, 6.1.1.23]; 6.1.1.12 [EC: 6.1.1.12, 6.1.1.23]

Predicted SEED Role

"Aspartyl-tRNA synthetase (EC 6.1.1.12)" in subsystem tRNA aminoacylation, Asp and Asn (EC 6.1.1.12)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.12 or 6.1.1.23

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (590 amino acids)

>B158DRAFT_1122 aspartyl-tRNA synthetase (EC 6.1.1.12) (Kangiella aquimarina DSM 16071)
MTSKMRSHYCGELSKNEVNQLVTLSGWVHRRRDLGGLIFLQVRDRSGLIQVVIDPDFKEL
FATAEQLRNEFVIQVKGQVRARPEEMVNKNMTTGEIEVLAQELTILNKAQPPAIPIDENL
EASEEQRLKYRYLDLRRPEMAQNLIFRHKVTSAMRRFMDEHGFIDVETPILTKATPEGAR
DYLVPSRVHKGSFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQLDI
ETSFLDQEEVMELMEGLIRHLFKDLKNIELPTFPRMSYAEAMERYGSDKPDLRTDMELVT
VDDIVKDVEFKVFAGPANDEQSRVAALRVPGGAQLSRKQIDEYTNYVGIYGAKGLAWMKI
NGQGAENLQSPILKFLGDDKALAIVEQVGAQEGDIIFFGSDSKRVVADAMGALRLKVAAD
LELIRDEWKPLWVVDFPMFEEIDGEIHALHHPFTAPKENDAAAVKANPTETLSKAYDMVL
NGSEVGGGSIRIHNQDMQETVFDILGIDETEQKEKFGFLLDALKFGCPPHGGLAFGLDRL
VMLMVGADSIRDVIAFPKTTTASCPLTQAPSIVEEKQLDELGIQVKKKEQ