Protein Info for B158DRAFT_0608 in Kangiella aquimarina DSM 16071

Annotation: chaperone protein DnaK

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 643 TIGR02350: chaperone protein DnaK" amino acids 3 to 602 (600 residues), 972.5 bits, see alignment E=3.7e-297 PF00012: HSP70" amino acids 4 to 602 (599 residues), 899.2 bits, see alignment E=1.1e-274 PF06723: MreB_Mbl" amino acids 116 to 375 (260 residues), 40.1 bits, see alignment E=2.1e-14

Best Hits

Swiss-Prot: 78% identical to DNAK_MARHV: Chaperone protein DnaK (dnaK) from Marinobacter hydrocarbonoclasticus (strain ATCC 700491 / DSM 11845 / VT8)

KEGG orthology group: K04043, molecular chaperone DnaK (inferred from 98% identity to kko:Kkor_2396)

MetaCyc: 74% identical to chaperone protein DnaK (Escherichia coli K-12 substr. MG1655)
Non-chaperonin molecular chaperone ATPase. [EC: 3.6.4.10, 5.6.1.7]

Predicted SEED Role

"Chaperone protein DnaK" in subsystem Heat shock dnaK gene cluster extended or Protein chaperones

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.6.4.10 or 5.6.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (643 amino acids)

>B158DRAFT_0608 chaperone protein DnaK (Kangiella aquimarina DSM 16071)
MAKIIGIDLGTTNSCVAVMDGDKPRVIENSEGARTTPSIIAFTDGETLVGQSAKRQAVTN
PQNTLFAIKRLIGRKFKDDVVQKDKGMVPYKIIEADNGDAWVEANGKKMAPPQISAEVLK
KMKKTAEDFLGEEVKEAVITVPAYFNDSQRQATKDAGRIAGLDVKRIINEPTAAALAYGM
DKERGDRTIAVYDLGGGTFDISIIEIAEVDGEHTFEVLSTNGDTFLGGEDFDMRVIEYLA
AEFKKDSGIDLKGDALAMQRLKEAGEKAKIELSSSTQTEINLPYITADATGPKHLNLKLT
RAKLEALVEDLIERSMEPVKQALKDAGLSTSEINDVILVGGQTRMPKVQEAVAGFFGKDP
RKDVNPDEAVAVGAAIQGAVLSGDVKDVLLLDVTPLSLGIETMGGVMTPLIEKNTTIPTK
ASQTFSTAEDNQTAVTVHVLQGERKMASENKSLGRFDLADIPPAPRGMPQIEVTFDIDAN
GIMHVSAKDKATGKEQSIQIKASSGLSDEEVEQMIKDAEAHAEEDKKFQELVEARNQADG
LVHATRKSLKDENITFEDGEKEAIEKAAEELEEAIKGNDIEDIKAKTEALSQASAKLAER
MYANAQAQAEAGQQAGGEQAQGQGAADDVVDAEFTEVKDEDKK