Protein Info for B158DRAFT_0518 in Kangiella aquimarina DSM 16071

Annotation: Phosphoglycerate dehydrogenase and related dehydrogenases

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00389: 2-Hacid_dh
  • amino acids 33 to 346 (314 residues), 120.8 bits, E=5.2e-39
• PF02826: 2-Hacid_dh_C
  • amino acids 138 to 314 (177 residues), 174.6 bits, E=2.1e-55
• PF03446: NAD_binding_2
  • amino acids 174 to 277 (104 residues), 22.9 bits, E=1.2e-08

Best Hits

Swiss-Prot: 62% identical to SERA_ECO57: D-3-phosphoglycerate dehydrogenase (serA) from Escherichia coli O157:H7

KEGG orthology group: K00058, D-3-phosphoglycerate dehydrogenase [EC: 1.1.1.95] (inferred from 93% identity to kko:Kkor_2479)

MetaCyc: 62% identical to phosphoglycerate dehydrogenase (Escherichia coli K-12 substr. MG1655)
Phosphoglycerate dehydrogenase. [EC: 1.1.1.95]; KETOGLUTREDUCT-RXN [EC: 1.1.1.95, 1.1.1.399]; 1.1.1.399 [EC: 1.1.1.95, 1.1.1.399]

Predicted SEED Role

"D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95)" in subsystem Glycine and Serine Utilization or Pyridoxin (Vitamin B6) Biosynthesis or Serine Biosynthesis (EC 1.1.1.95)

MetaCyc Pathways

• superpathway of sulfate assimilation and cysteine biosynthesis (9/9 steps found)
• superpathway of L-serine and glycine biosynthesis I (4/4 steps found)
• L-serine biosynthesis I (3/3 steps found)
• L-cysteine biosynthesis IX (Trichomonas vaginalis) (2/3 steps found)
• L-glutamate degradation V (via hydroxyglutarate) (5/10 steps found)
• L-glutamate degradation XI (reductive Stickland reaction) (2/7 steps found)

KEGG Metabolic Maps

• Glycine, serine and threonine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.95

Use Curated BLAST to search for 1.1.1.399 or 1.1.1.95

Protein Sequence (429 amino acids)

>B158DRAFT_0518 Phosphoglycerate dehydrogenase and related dehydrogenases (Kangiella aquimarina DSM 16071)
MLIGSVLIDLRCLLTAEFIKMANTSFDKHQLKVLLLEGVHPKAEQLFRQDGYHQVESLKT
ALSASELKSALQDCHFLGIRSRSQITPEVLAEANKLVAIGCFCIGTNQVNLTAAQHKGIP
VFNAPYSNTRSVAELVLGEIIMLLRGIPAKNAAAHRGQWLKTAQNSNEARGKTLGIIGYG
HIGTQLGILAEGIGMQVKFYDIETKLTLGNASQVSDLNQLLAESDVVTLHVPETPQTKGM
MGKAELAAMKSGSILINASRGTVVDIDALTEALRSEHILGAAIDVFPTEPKSNTEEFQSP
LRDFDNVILTPHIGGSTLEAQENIAIEVTEKMIKYSNNGSTVSAVNFPQVALPEHINTHR
ILHIHHNKPGILSQINQILSQNDINISAQYLMTQGDIGYVVTDIDQSSSHFALEKMREIE
GTIRARVLY