Protein Info for B158DRAFT_0352 in Kangiella aquimarina DSM 16071

Annotation: ATP-dependent DNA helicase Rep (EC 3.6.1.-)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 688 signal peptide" amino acids 1 to 17 (17 residues), see Phobius details TIGR01074: ATP-dependent DNA helicase Rep" amino acids 19 to 685 (667 residues), 1009.2 bits, see alignment E=4e-308 PF00580: UvrD-helicase" amino acids 20 to 283 (264 residues), 311.2 bits, see alignment E=1.9e-96 PF13245: AAA_19" amino acids 24 to 266 (243 residues), 86.3 bits, see alignment E=5.5e-28 PF13361: UvrD_C" amino acids 288 to 632 (345 residues), 252.1 bits, see alignment E=2.7e-78 PF13538: UvrD_C_2" amino acids 573 to 627 (55 residues), 28.5 bits, see alignment 2.8e-10

Best Hits

Swiss-Prot: 59% identical to REP_SALTY: ATP-dependent DNA helicase Rep (rep) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K03656, ATP-dependent DNA helicase Rep [EC: 3.6.4.12] (inferred from 95% identity to kko:Kkor_0017)

MetaCyc: 58% identical to ATP-dependent DNA helicase Rep (Escherichia coli K-12 substr. MG1655)
RXN-11135 [EC: 5.6.2.4]

Predicted SEED Role

"ATP-dependent DNA helicase Rep" in subsystem DNA repair, bacterial UvrD and related helicases

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.12

Use Curated BLAST to search for 3.6.4.12 or 5.6.2.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (688 amino acids)

>B158DRAFT_0352 ATP-dependent DNA helicase Rep (EC 3.6.1.-) (Kangiella aquimarina DSM 16071)
MLNLPPVLINLFAALSLPQLNEKQREALLATQGPLLVLAGAGSGKTSVITRKMVYLIKEK
QIPARNIVAVTFTNKAAREMKQRVSKLAGSNATQGLTVSTFHNFGLNFIRREYKKLGMKS
NFTIFDDQDTLALLKDLGFKEAESDKQLLFRLQQQISAWKNDLILPEQAINQVKDQEQLI
FSKVYEKYARSMKAYNAVDFDDLILIPTLLLRDDPEVREKWQNKIRYLLVDEYQDTNTSQ
YELIKHLVGQFGSFTVVGDDDQSIYSWRGARPENLDQLAKDYPNLQVIKLEQNYRSYGRI
LKAANVLIDNNPHVFEKKLWSDKPYGDPLKVVTCPNEDAEAEKVVTEIISRKVRTKNGRY
SDFAILYRGNHQAKIFERTLIANNVPYKISGSTSFFSRAEIKDIMAYLRLLTNPDDDNAF
LRIANTPRRGIGPTTLEQLGSYANMRHISLFAATQEIGLEQHLQGKGLENIRRFADLISR
AADNAERGDSVGVIHDLVARIGYHSWLHETSSTPKAAEYRWNNIQELLGWVEKGIDDNEH
DPNPFAAAVANLMLRDMLDRNEKEEEESNEVQLMTLHAAKGLEFNHVYLVGMEEEFLPHK
SSIENDDIEEERRLAYVGITRAQQTLTFTLCKKRSKYGEVMACEPSRFLEELPQDDLDWD
AKRKPLSEEEQEQLATDNISMLKSLFAD