Protein Info for B158DRAFT_0167 in Kangiella aquimarina DSM 16071

Annotation: phosphoribosylamine--glycine ligase (EC 6.3.4.13)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 427 PF02844: GARS_N" amino acids 1 to 102 (102 residues), 131.9 bits, see alignment E=3.5e-42 TIGR00877: phosphoribosylamine--glycine ligase" amino acids 1 to 421 (421 residues), 605 bits, see alignment E=3.4e-186 PF01071: GARS_A" amino acids 103 to 296 (194 residues), 276.1 bits, see alignment E=4.1e-86 PF02655: ATP-grasp_3" amino acids 104 to 291 (188 residues), 25.7 bits, see alignment E=2.8e-09 PF02786: CPSase_L_D2" amino acids 109 to 247 (139 residues), 26.2 bits, see alignment E=1.4e-09 PF02843: GARS_C" amino acids 331 to 419 (89 residues), 124.8 bits, see alignment E=3.5e-40

Best Hits

Swiss-Prot: 73% identical to PUR2_VIBPA: Phosphoribosylamine--glycine ligase (purD) from Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)

KEGG orthology group: K01945, phosphoribosylamine--glycine ligase [EC: 6.3.4.13] (inferred from 91% identity to kko:Kkor_0247)

MetaCyc: 72% identical to phosphoribosylamine--glycine ligase (Escherichia coli K-12 substr. MG1655)
Phosphoribosylamine--glycine ligase. [EC: 6.3.4.13]

Predicted SEED Role

"Phosphoribosylamine--glycine ligase (EC 6.3.4.13)" in subsystem De Novo Purine Biosynthesis (EC 6.3.4.13)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.4.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (427 amino acids)

>B158DRAFT_0167 phosphoribosylamine--glycine ligase (EC 6.3.4.13) (Kangiella aquimarina DSM 16071)
MNVLIIGSGGREHALAWKAAQSSEVDTVFVAPGNAGIALESKIKTVAIGVEDIDALIDFA
KGNQVALTIVGPEAPLVIGVVDAFTQAGLKCFGPSKGAAQLEGSKAFTKDFLARHNIPTA
AYQNFTEIEPAKAYVREMGTPIVIKADGLAAGKGVIIAQTTDEADAAIDDMLAGNKFGDA
GHRVVIEEFLQGEEASFIVMVDGKNILPLATSQDHKARDNGDTGPNTGGMGAYSPAPVVT
PEMHDRIMQQVIIPTVEGMAAEGHPYTGFLYAGVMIDADGVPKVLEYNCRFGDPETQPIM
SRLQSDLVGLCLTALDGKLDTATAEWDSRAAVGVVMAAGGYPDSYHKGDVIHGLDSVSGA
KVFHAGTTEKDGQIVTNGGRVLCVVALGDTVTEAQAKAYEGVKQINWDNVYYRTDIGHRA
IAREQSK