Protein Info for B158DRAFT_0058 in Kangiella aquimarina DSM 16071

Annotation: poly(A) polymerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 456 TIGR01942: poly(A) polymerase" amino acids 28 to 453 (426 residues), 517.5 bits, see alignment E=1.3e-159 PF01743: PolyA_pol" amino acids 60 to 184 (125 residues), 131.6 bits, see alignment E=3.2e-42 PF12627: PolyA_pol_RNAbd" amino acids 212 to 272 (61 residues), 63.5 bits, see alignment E=1.9e-21 PF12626: PolyA_pol_arg_C" amino acids 331 to 444 (114 residues), 133.9 bits, see alignment E=4.3e-43

Best Hits

Swiss-Prot: 51% identical to PCNB_ECO57: Poly(A) polymerase I (pcnB) from Escherichia coli O157:H7

KEGG orthology group: K00970, poly(A) polymerase [EC: 2.7.7.19] (inferred from 90% identity to kko:Kkor_0354)

MetaCyc: 51% identical to poly(A) polymerase I (Escherichia coli K-12 substr. MG1655)
Polynucleotide adenylyltransferase. [EC: 2.7.7.19]

Predicted SEED Role

"Poly(A) polymerase (EC 2.7.7.19)" in subsystem Polyadenylation bacterial (EC 2.7.7.19)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.19

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (456 amino acids)

>B158DRAFT_0058 poly(A) polymerase (Kangiella aquimarina DSM 16071)
MSSIIKSVKRWFASPAVESSGSSNRTQIISRDQHSISRKDISENALKVLYRLSNAGYESF
LVGGGVRDLLLGLHPKDFDIATNATPEQVKELFRNCRIVGRRFRLAHILFGRDVIEVATF
RAHCDDNIQQAKEGMILRDNVYGDIEEDAIRRDFTINALYYNIKDFSVYDYCDGLSDLRK
RQLRMIGDPETRYREDPVRMLRVVRLACKLDLSIEPKTLKPVSELSYLLEQVPAARLWEE
YRKLFLAGAGKLTFEKLREYGLFHALFPATDRSLKHDDTAQAQQTEQLILDALENTDTRI
ATEQTVNPAFLMAVMLWQPLLESTEALQQKGNNFNDAFFKSMARVLNAQSQHISIPRRYA
LVIRDIWSMQLRLPAHHGKKAWSIFEHPRFRAAYDFLLLRSQSDPRQLELANWWTKFQDA
DANARRQMLQQTGGGKKRRRRKPRNPKRQQASKNRH