Protein Info for Atu6022 in Agrobacterium fabrum C58

Annotation: N-methylhydantoinase A/acetone carboxylase, beta subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 678 PF05378: Hydant_A_N" amino acids 3 to 181 (179 residues), 176.4 bits, see alignment E=6.8e-56 PF01968: Hydantoinase_A" amino acids 203 to 487 (285 residues), 302.2 bits, see alignment E=5.7e-94 PF19278: Hydant_A_C" amino acids 530 to 667 (138 residues), 54.9 bits, see alignment E=1.2e-18

Best Hits

KEGG orthology group: K01473, N-methylhydantoinase A [EC: 3.5.2.14] (inferred from 100% identity to atu:Atu6022)

Predicted SEED Role

"N-methylhydantoinase A (EC 3.5.2.14)" in subsystem Hydantoin metabolism (EC 3.5.2.14)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.2.14

Use Curated BLAST to search for 3.5.2.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A9CL05 at UniProt or InterPro

Protein Sequence (678 amino acids)

>Atu6022 N-methylhydantoinase A/acetone carboxylase, beta subunit (Agrobacterium fabrum C58)
MLRIGVDIGGTFTDFCGWREGEDRIVTLKVPSTPPAFENGFREGFEKLLERLAPEPGEAA
FLMHGTTVSTNAVIERKGPKIAFFVTKGYRDLLELQRIGVRNPLNLFETRTRPLIDREMV
FEVEERLLRGGLVRTSIDEAAVEALARRAADAGAAGYAVALLHSYANPQHEQAVARAIRK
AVGEDAEVSLSSEIWPRIGEYERAIVSVLNAFVKRRMNEYIGAVETYVAERLPGSQLFVT
RSNGGAMAASEARNFPVHTLLSGPASGVTAVQYLGRALGEHNILTMDMGGTSTDISLVRD
GEALTSTSAEVGEFPVVMPVTSIEAIGAGGGSVVTIDGGVLRIGPRSAGSYPGPACFSRG
GTEPTLTDAFLLAGYLPEALLGGDMQLDRHAAERAMAPIAETLKTDVLSAAEMCVTVASS
NMVAGVLPYLARQGVDPEDLTVLVYGGAGAIQGPLLAAEIGVNRVLVPATPSVFCALGGL
VSDLSNDALETVHGLDIDGSLIAKKFQVLREQGAEWLSRQAPPERLVSKTFECWAEMRYV
GQSFQVDVRLPDTAIEAHDIAAMHAAFHQEHERIYSHADKAAPVEFVDLRMRVRGSMSIP
QPSTPETSGAGGALKTVRSMRFHNKLIPEVRVFDRASLTPNDAIQGPAVIEQRDATIVVP
PEFVARVGAFGAILMTRS