Protein Info for Atu5535 in Agrobacterium fabrum C58

Annotation: ABC transporter nucleotide binding/ATPase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 231 TIGR03410: urea ABC transporter, ATP-binding protein UrtE" amino acids 2 to 231 (230 residues), 311.1 bits, see alignment E=2.2e-97 PF00005: ABC_tran" amino acids 17 to 159 (143 residues), 100.9 bits, see alignment E=9.2e-33

Best Hits

Swiss-Prot: 38% identical to LIVF_METJA: Probable branched-chain amino acid transport ATP-binding protein LivF (livF) from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)

KEGG orthology group: K01996, branched-chain amino acid transport system ATP-binding protein (inferred from 100% identity to atu:Atu5535)

MetaCyc: 42% identical to branched chain amino acid/phenylalanine ABC transporter ATP binding subunit LivF (Escherichia coli K-12 substr. MG1655)
ABC-15-RXN [EC: 7.4.2.2]; 7.4.2.2 [EC: 7.4.2.2]; 7.4.2.2 [EC: 7.4.2.2]; 7.4.2.2 [EC: 7.4.2.2]

Predicted SEED Role

"Urea ABC transporter, ATPase protein UrtE" in subsystem Urea decomposition

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.4.2.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A9CL24 at UniProt or InterPro

Protein Sequence (231 amino acids)

>Atu5535 ABC transporter nucleotide binding/ATPase (Agrobacterium fabrum C58)
MLTLKNVHSYYGRSHILHGVTLDVPTGKVTSILGRNSTGKTTLLKTLMALTDGMTGEMHL
QGQDISSSPTHMRARAGIAYVPQGREIIPDFSIRENILMGAFARADRKREIPALVPELFP
YLMANLDRPGGVLSGGQQQQLAIARALAADPKVLLLDEPNEGIQPSIVEEIEKIIIRLNR
EIGMTIVLVEQNVAFARHASHHFAMLEKGGVVAAGAIDALSDTLVHRHMAV