Protein Info for Atu5528 in Agrobacterium fabrum C58

Annotation: two component sensor kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 642 PF08448: PAS_4" amino acids 101 to 197 (97 residues), 31.6 bits, see alignment E=7.4e-11 amino acids 215 to 323 (109 residues), 43.5 bits, see alignment E=1.6e-14 amino acids 338 to 445 (108 residues), 60.9 bits, see alignment E=6.1e-20 TIGR00229: PAS domain S-box protein" amino acids 204 to 327 (124 residues), 86 bits, see alignment E=1.1e-28 amino acids 326 to 448 (123 residues), 104 bits, see alignment E=3.1e-34 PF00989: PAS" amino acids 208 to 315 (108 residues), 40.5 bits, see alignment E=1.1e-13 amino acids 331 to 441 (111 residues), 72.1 bits, see alignment E=1.7e-23 PF13188: PAS_8" amino acids 208 to 261 (54 residues), 30.5 bits, see alignment 1.1e-10 PF13426: PAS_9" amino acids 219 to 320 (102 residues), 58.4 bits, see alignment E=3.4e-19 amino acids 341 to 443 (103 residues), 59.9 bits, see alignment E=1.2e-19 PF08447: PAS_3" amino acids 231 to 313 (83 residues), 48.8 bits, see alignment E=3.1e-16 amino acids 353 to 437 (85 residues), 34.6 bits, see alignment E=8.8e-12 PF13596: PAS_10" amino acids 334 to 442 (109 residues), 25.2 bits, see alignment E=9.1e-09 PF07536: HWE_HK" amino acids 457 to 535 (79 residues), 82.2 bits, see alignment E=1.7e-26 PF07568: HisKA_2" amino acids 457 to 520 (64 residues), 23.2 bits, see alignment E=2.6e-08

Best Hits

KEGG orthology group: None (inferred from 100% identity to atu:Atu5528)

Predicted SEED Role

"Chemotaxis protein methyltransferase CheR (EC 2.1.1.80)" in subsystem Bacterial Chemotaxis (EC 2.1.1.80)

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.80

Use Curated BLAST to search for 2.1.1.80

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q7D2U9 at UniProt or InterPro

Protein Sequence (642 amino acids)

>Atu5528 two component sensor kinase (Agrobacterium fabrum C58)
MATSHASARTYETRCNRGELLARSGKFYKLQPVTPEVEILILNNVNEPANAVPVASLLDV
TDWASGPLGPRSEWPACLHAAIGIMLPSQAQIVMFWGQEFVALYNDIYAPTIGNKHPHAF
GRPAREYWNELWDDLEPLLKRVLDKGETIAAKDRPFYIERHGYPETVYFDISYSPVTDQD
GIVRGVFCIVNETTERVKADAALRESEERLRAIFAQSAVGIALGDLTGKLISVNDHFCQI
VGRRRDELIGIQMQDITFAEDLPENQRLFKHMVETGESFEIEKRYVRGDGSLVWVLNSVS
AIRDDQGNMSQAVAISADIGERRHAQEVERHLASMIASSNDAILGIDLDMTITSWNAAAE
KLYGYSEDEVVGRTVLMLVPDDRQEEEPTILRQIKAGRIVEPYETQRLRKDGRLVEVLLS
VSPIRDVNGRVIGASKTAHDITARKDADRLRSILVNELHHRVKNILATVTAIARQTIGRD
KANHEDMEAFTSRLGSLSRAQDLLVHADWQHADLRAVLQQALSPYPAESFQISGPGVSLP
PRAVVSLSLALHELATNAAKYGALSVPEGQVSISWQRQGTDGERLRVVWEERGGPEVMSP
QRKGFGSTLVERLLSAELNGETKLFYEKNGVICVIEAEINHV