Protein Info for Atu5527 in Agrobacterium fabrum C58

Annotation: NADH-dependent FMN reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 PF03358: FMN_red" amino acids 5 to 149 (145 residues), 102.5 bits, see alignment E=1.7e-33 PF02525: Flavodoxin_2" amino acids 5 to 172 (168 residues), 44.8 bits, see alignment E=1.2e-15 TIGR03566: FMN reductase" amino acids 6 to 178 (173 residues), 240.1 bits, see alignment E=6e-76

Best Hits

Swiss-Prot: 45% identical to SFNF_PSEPF: NADH-dependent FMN reductase SfnF (sfnF) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K00299, FMN reductase [EC: 1.5.1.29] (inferred from 100% identity to atu:Atu5527)

Predicted SEED Role

"FMN reductase (EC 1.5.1.29)" in subsystem Alkanesulfonate assimilation or Alkanesulfonates Utilization (EC 1.5.1.29)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.5.1.29

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A9CL27 at UniProt or InterPro

Protein Sequence (200 amino acids)

>Atu5527 NADH-dependent FMN reductase (Agrobacterium fabrum C58)
MRRTMNVLGISGSVKQPSRTARVVSDILASIERRASVSVDTIDLAVAAPILFRALRADQL
DEAGRRIIDAVEIADVLVVGSPVYRASYTGALKHLFDLVDFRALQGKRVVLAATGGTPLH
GLMLEHQFRPLFGFFGAVTVPTTVYAVEADFTGYTLSNPEVEARIERAVAELIDLLPARD
VAAADHHSALRVVSNEAVAN