Protein Info for Atu5469 in Agrobacterium fabrum C58

Annotation: DNA polymerase IV

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 357 PF00817: IMS" amino acids 7 to 153 (147 residues), 184.6 bits, see alignment E=1.6e-58 PF11798: IMS_HHH" amino acids 165 to 196 (32 residues), 26.1 bits, see alignment (E = 1.1e-09) PF11799: IMS_C" amino acids 239 to 355 (117 residues), 99.2 bits, see alignment E=2.8e-32

Best Hits

Swiss-Prot: 100% identical to DPO42_AGRFC: DNA polymerase IV 2 (dinB2) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K02346, DNA polymerase IV [EC: 2.7.7.7] (inferred from 100% identity to atu:Atu5469)

Predicted SEED Role

"DNA polymerase IV (EC 2.7.7.7)" in subsystem DNA repair, bacterial (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8UJK7 at UniProt or InterPro

Protein Sequence (357 amino acids)

>Atu5469 DNA polymerase IV (Agrobacterium fabrum C58)
MRKIIHVDMDAFYASVEQRDNPELRGRPVAVGSAAARGVVAAASYEAREFGVRSAMPSVT
AARRCPDLIFVPPRFDVYKAVSQQIRAIFAEYTRLIEPLSLDEAYLDVTENLKGMEIATE
IASEIRERIKQITGLNASAGISYNKFLAKMASDLNKPNGQAVITPKNGPAFVEQLAVKKF
HGVGPATAEKMHRFGIETGADLKSKSLQFLAEHFGKSGAYFYGIARGIDERQVRPDRIRK
SVGAEDTFSVDINDLDLATAELRPLAEKVWHHCEAQRVSGKTVTVKVKYSDFTQATRSRT
SALPVNGIQEILEAASALLATVYPFRRSVRLLGVTLSSLTNDQEAEDEEQPQLDLAL