Protein Info for Atu4734 in Agrobacterium fabrum C58

Annotation: bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF11791: Aconitase_B_N
  • amino acids 6 to 162 (157 residues), 142.7 bits, E=1.5e-45
• PF06434: Aconitase_2_N
  • amino acids 175 to 406 (232 residues), 212.9 bits, E=6e-67
• PF00330: Aconitase
  • amino acids 411 to 894 (484 residues), 172.4 bits, E=2.6e-54

Best Hits

KEGG orthology group: K01682, aconitate hydratase 2 [EC: 4.2.1.3] (inferred from 100% identity to atu:Atu4734)

Predicted SEED Role

"Aconitate hydratase 2 (EC 4.2.1.3)" in subsystem Propionate-CoA to Succinate Module or TCA Cycle (EC 4.2.1.3)

MetaCyc Pathways

• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (20/22 steps found)
• superpathway of glyoxylate bypass and TCA (12/12 steps found)
• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (22/26 steps found)
• TCA cycle I (prokaryotic) (10/10 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (12/14 steps found)
• TCA cycle III (animals) (9/10 steps found)
• glyoxylate cycle (6/6 steps found)
• TCA cycle II (plants and fungi) (8/9 steps found)
• TCA cycle IV (2-oxoglutarate decarboxylase) (8/9 steps found)
• TCA cycle V (2-oxoglutarate synthase) (8/9 steps found)
• TCA cycle VII (acetate-producers) (8/9 steps found)
• partial TCA cycle (obligate autotrophs) (7/8 steps found)
• TCA cycle VI (Helicobacter) (6/9 steps found)
• nitrogen remobilization from senescing leaves (5/8 steps found)
• reductive TCA cycle I (7/11 steps found)
• methylaspartate cycle (12/19 steps found)
• ethene biosynthesis V (engineered) (16/25 steps found)
• mixed acid fermentation (9/16 steps found)
• reductive TCA cycle II (6/12 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Glyoxylate and dicarboxylate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.3

Use Curated BLAST to search for 4.2.1.3

Sequence Analysis Tools

See Q7CVQ5 at UniProt or InterPro

Protein Sequence (929 amino acids)

>Atu4734 bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase (Agrobacterium fabrum C58)
MNLYLDYLAEIKSRETLGLAPKPIDDGALTAEIIELIEDTSSEYRADALKFFIYNTLPGT
TSAAGVKAAFLKQIILGEVLVPEITPSFALELLSHMKGGPSIKVLLDVALGNDAAIDAKA
GEVLKTQVFLYDADMFRLRDAYKEGSAIARDVLESYAKAEFFTKLPDVDDEIKIVTFIAA
EGDISTDLLSPGNQAHSRSDRQLHGQCMISPEAQAEIVALQQQHPDKRVMLIAEKGTMGV
GSSRMSGVNNVALWTGKQASPYVPFVNYAPIVAGTNGISPIFATTVDVTGGIGINLKNWV
KKLGEDGKPILNNDGNPILEQKYSVETGTVLKIDAKNRKLRDENGTELVDLASSFTPQKM
EFMKAGSSYAIVFGKKLQTFAAQTLGVEATQVFAPNKEISIEGQGLTAVEKIFNRNAVGV
TPGKVLHAGSDVRVKVNIVGSQDTTGLMTAQELEAMAATVISPLVDGAYQSGCHTASVWD
KKAQANTPKLMSFMHNFGVITGRDPKGVYHSMTDVIHKVLNDITVDDRAIIIGGDSHTRM
SKGVAFGADSGTVALALATGEATMPIPQSVKVTFKGTMQPYMDFRDVVHATQAQMLQQHG
DNVFQGRIIEVHIGTLLADQAFTFTDWTAEMKAKASICISEDETLIESLEIAKSRIQIMI
DKGMDNAAQTLKGLIDKADQRIAEIRSGETPALKPDANAKYFAEVVVDLDIINEPMIADP
DVNNNDVSRRYTHDTIRPVSYYGATKKVDLGFVGSCMVHKGDVKIVAQMLRNMEKTEGKV
EFKAPLVVAAPTYNIIDELKAEGDWEILQKYSGFEFDDVNPKTSNRTEYDNILYLERPGC
NLCMGNQEKAAKGDTVLATSTRLFQGRVVEDSAEKKGESLLASTPVVVLSAILGRTPSIE
EYRSAVEGIDLTKFAPPKTTPIDSQSVHY