Protein Info for Atu4691 in Agrobacterium fabrum C58

Annotation: 2-hydroxyacid dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00389: 2-Hacid_dh
  • amino acids 37 to 310 (274 residues), 70.3 bits, E=1.9e-23
• PF02826: 2-Hacid_dh_C
  • amino acids 108 to 279 (172 residues), 181.5 bits, E=1.6e-57
• PF03446: NAD_binding_2
  • amino acids 146 to 244 (99 residues), 21.8 bits, E=2.6e-08

Best Hits

Swiss-Prot: 52% identical to HPR2_ARATH: Glyoxylate/hydroxypyruvate reductase A HPR2 (HPR2) from Arabidopsis thaliana

KEGG orthology group: None (inferred from 100% identity to atu:Atu4691)

MetaCyc: 52% identical to glyoxylate reductase (NADP+) [multifunctional] (Arabidopsis thaliana col)
Hydroxypyruvate reductase. [EC: 1.1.1.81]; Glyoxylate reductase (NADP(+)). [EC: 1.1.1.81, 1.1.1.79]

Predicted SEED Role

"D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95)" in subsystem Glycine and Serine Utilization or Pyridoxin (Vitamin B6) Biosynthesis or Serine Biosynthesis (EC 1.1.1.95)

MetaCyc Pathways

• superpathway of sulfate assimilation and cysteine biosynthesis (9/9 steps found)
• superpathway of L-serine and glycine biosynthesis I (4/4 steps found)
• L-serine biosynthesis I (3/3 steps found)
• L-cysteine biosynthesis IX (Trichomonas vaginalis) (2/3 steps found)
• L-serine biosynthesis II (1/4 steps found)
• formaldehyde assimilation I (serine pathway) (5/13 steps found)

KEGG Metabolic Maps

• Glycine, serine and threonine metabolism
• Glyoxylate and dicarboxylate metabolism
• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.79, 1.1.1.81, 1.1.1.95

Use Curated BLAST to search for 1.1.1.79 or 1.1.1.81 or 1.1.1.95

Sequence Analysis Tools

See A9CH04 at UniProt or InterPro

Protein Sequence (311 amino acids)

>Atu4691 2-hydroxyacid dehydrogenase (Agrobacterium fabrum C58)
MTAEIVQLCPLIPALEEELAQRFTVHRLFEAADKAAFLSEKGAAIRGVVTGGHIGLPADV
GAALPNLEIVAINGVGFDKVDLGEAKRRGFRVSNTPDVLTADVADLALGLVLAQARKVPQ
ADQHVRTGQWLKGDMGLSTRVAGRRYGIFGLGRIGQAIAKRLEGFDARISYTARNRRDVA
YDYHDSIEELAANCDVLIIAAAATAETRHIVNADVLKALGPQGVLVNVARGSLVDEKALV
EALSSGMIGGAALDVFEDEPRVPEALFAFENVTLAPHIGSGTHQTRRAMADLVLANLDAH
FAGRELPTPVV