Protein Info for Atu4675 in Agrobacterium fabrum C58

Annotation: dihydrodipicolinate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 321 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details PF00701: DHDPS" amino acids 6 to 242 (237 residues), 107.6 bits, see alignment E=2.6e-35

Best Hits

KEGG orthology group: K01714, dihydrodipicolinate synthase [EC: 4.2.1.52] (inferred from 100% identity to atu:Atu4675)

MetaCyc: 75% identical to Delta1-pyrroline-4-hydroxy-2-carboxylate deaminase subunit (Pseudomonas putida KT2442)
1-pyrroline-4-hydroxy-2-carboxylate deaminase. [EC: 3.5.4.22]

Predicted SEED Role

"1-pyrroline-4-hydroxy-2-carboxylate deaminase (EC 3.5.4.22)" in subsystem Proline, 4-hydroxyproline uptake and utilization (EC 3.5.4.22)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.52

Use Curated BLAST to search for 3.5.4.22 or 4.2.1.52

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A9CGZ4 at UniProt or InterPro

Protein Sequence (321 amino acids)

>Atu4675 dihydrodipicolinate synthase (Agrobacterium fabrum C58)
MTASIFSGVIPALMTPCRQDRTPDFDALVRKGKELIADGMSAVVYCGSMGDWPLLTDEQR
MEGVERLVKAGIPVIVGTGAVNTASAVAHAVHAQKVGAKGLMVIPRVLSRGSVIAAQKAH
FKAILSAAPEIPAVIYNSPYYGFATRADLFFALRAEHKNLVGFKEFGGPADMRYAAENIT
SRDDEVTLMIGVDTAVVHGFVNCGATGAITGIGNVLPKEVIHLCKLSQAAAKGDADARAR
ALELEQALAVLSSFDEGPDLVLYFKYMMVLKGDKEYTLHFNETDALTDSQRGYVEAQFKL
FNSWYADWSKLPGAVQTCKAA