Protein Info for Atu4651 in Agrobacterium fabrum C58
Annotation: argininosuccinate lyase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to ARLY2_AGRFC: Argininosuccinate lyase 2 (argH2) from Agrobacterium fabrum (strain C58 / ATCC 33970)
KEGG orthology group: K01755, argininosuccinate lyase [EC: 4.3.2.1] (inferred from 100% identity to atu:Atu4651)Predicted SEED Role
"Argininosuccinate lyase (EC 4.3.2.1)" in subsystem Arginine Biosynthesis extended (EC 4.3.2.1)
MetaCyc Pathways
- L-arginine biosynthesis II (acetyl cycle) (10/10 steps found)
- L-arginine biosynthesis I (via L-ornithine) (9/9 steps found)
- superpathway of arginine and polyamine biosynthesis (14/17 steps found)
- L-arginine biosynthesis III (via N-acetyl-L-citrulline) (8/9 steps found)
- urea cycle (4/5 steps found)
- superpathway of L-citrulline metabolism (9/12 steps found)
- nitric oxide biosynthesis II (mammals) (2/3 steps found)
- canavanine biosynthesis (2/4 steps found)
- L-arginine biosynthesis IV (archaea) (4/9 steps found)
KEGG Metabolic Maps
- Alanine and aspartate metabolism
- Arginine and proline metabolism
- Urea cycle and metabolism of amino groups
Isozymes
Compare fitness of predicted isozymes for: 4.3.2.1
Use Curated BLAST to search for 4.3.2.1
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q8U705 at UniProt or InterPro
Protein Sequence (456 amino acids)
>Atu4651 argininosuccinate lyase (Agrobacterium fabrum C58) MLRETGILDAETAAKIAGALEDIDRTIEPSELVYTGEVEDFFFLIEKELKARIGVDVAGR LHTARSRNDIDHTLFKIGLKDKIDTLTAKARVLLKALIDAAERNQSTLIVAYTHGQPAQP TTFGHYLSAAIEVLIRDIERFTEARHIVDLSPMGAAAITTSGFPIDRARVAELLGFAAPL RNSYSCIAAVDYTTATYGAIELMFLHLGRLIQDFQFWTSFEVGQIYVPNSLVQISSIMPQ KRNPVPIEHLRHLASQTFGRARTMLDVMHNTPFTDMNDSEGETQSMGYEAFASAGRVLDL LASLVGQISIDPERVDQNIRRSCITITELADSLVRIEDLSFRQAHEIAATVAKSVVALKG DLPNDGYQPFLGAFSGLTGRETGIDEEKFRQIVSPEHFVAVRSRFGGPAPEPMREAFAAY RGKLGAFEAEAQRSTNHEAAKAAELAEKFTALTGAR