Protein Info for Atu4648 in Agrobacterium fabrum C58

Annotation: choline sulfatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 503 TIGR03417: choline-sulfatase" amino acids 3 to 500 (498 residues), 894.3 bits, see alignment E=1.3e-273 PF00884: Sulfatase" amino acids 4 to 345 (342 residues), 134 bits, see alignment E=1.5e-42 PF01663: Phosphodiest" amino acids 7 to 292 (286 residues), 30.1 bits, see alignment E=8e-11 PF16347: SGSH_C" amino acids 296 to 437 (142 residues), 47.6 bits, see alignment E=4.4e-16 PF12411: Choline_sulf_C" amino acids 448 to 499 (52 residues), 74 bits, see alignment 1.3e-24

Best Hits

Swiss-Prot: 72% identical to BETC_RHIME: Choline-sulfatase (betC) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K01133, choline-sulfatase [EC: 3.1.6.6] (inferred from 100% identity to atu:Atu4648)

MetaCyc: 72% identical to choline sulfatase (Sinorhizobium meliloti Rm2011)
Choline-sulfatase. [EC: 3.1.6.6]

Predicted SEED Role

"Choline-sulfatase (EC 3.1.6.6)" in subsystem Choline Transport or Choline and Betaine Uptake and Betaine Biosynthesis (EC 3.1.6.6)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.6.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A9CGY3 at UniProt or InterPro

Protein Sequence (503 amino acids)

>Atu4648 choline sulfatase (Agrobacterium fabrum C58)
MPRPNILILMADQFNGTFFPDGPADFLHAPHLKALAERSVRFANTYTASPLCAPARASFM
SGQLPSRTRVYDNAAEFASDIPTYAHHLRAAGYYTGLSGKMHFVGPDQLHGFEERLTTDI
YPADFGWTPDYTKPGERIDWWYHNLGSVTGAGVAEITNQMEYDDEVAYHATRKLYDLSRR
LDDRPWCLTVSFTHPHDPYVARRKFWDLYEDCAALDPQSKAIAFEEQDPHSKRLLEACDH
EAFEITGEQIRRARRGYFANISYIDEKIGEILDVLNATRMEDTIILFLSDHGDMLGERGL
WFKMCFFEGSARVPLMIAAPGWQPALIDAPVSTLDVTPTLCGLAGLDITSLKRWTDGEDL
AALAAGTGGRGPVPMEYAAEGSITPLVAIRDGRYKLSVCEADPPLLYDLETDPQELTNLA
HDPAFSDVLSSLSAEMGRRWQLPVFDAAVRESQARRWVVYKALRNGAYYPWDYQPLQKAS
ERYMRNHMDLNVLEENQRFPRGE