Protein Info for Atu4519 in Agrobacterium fabrum C58

Annotation: branched amino acid ABC transporter substrate-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 390 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details PF13458: Peripla_BP_6" amino acids 22 to 364 (343 residues), 232.1 bits, see alignment E=2.3e-72 PF13433: Peripla_BP_5" amino acids 23 to 363 (341 residues), 98.4 bits, see alignment E=7.4e-32 PF01094: ANF_receptor" amino acids 59 to 348 (290 residues), 46 bits, see alignment E=6e-16

Best Hits

Swiss-Prot: 86% identical to LIVB6_BRUME: Leu/Ile/Val-binding protein homolog 6 (BMEII0633) from Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)

KEGG orthology group: K01999, branched-chain amino acid transport system substrate-binding protein (inferred from 100% identity to atu:Atu4519)

Predicted SEED Role

"Leucine-, isoleucine-, valine-, threonine-, and alanine-binding protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q7CV59 at UniProt or InterPro

Protein Sequence (390 amino acids)

>Atu4519 branched amino acid ABC transporter substrate-binding protein (Agrobacterium fabrum C58)
MKLRITAAIVAICFSSAAYAETIKVGVVGPFSGPFALQGKNFKAGIDAWFALHGNKVGND
TVEVVYRDVPQADPAQSKALAQELVVKERVQYLAGFYFTPDAMAVTPLLKQGNVPMVVMN
AATSAIVTKSPYVVRTSFTTWQTSTPIAKVAFDGGVKKVISLVSDYGPGVDAENAFKAAF
TAAGGEVVETIRMPLSTNDFSPIMQRVKDSGAQGVFAFLPSGPTTLGFVKAFNDNGLKSS
GVKLFAPGDLTQESDLPALGEAALGMQTTFHYAVSHDSPENKTFVEAAAKAIGNPKELSF
PAVSAFDGMYVISKMIEATGGKQDAEKAVEAVKGLSWTSPRGPVSIDPESRHITQNIYLR
EVAKADDGTYYNKEVQTFEKQGDPGLAAAK