Protein Info for Atu4516 in Agrobacterium fabrum C58

Annotation: branched chain amino acid ABC transporter ATPase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 254 PF00005: ABC_tran" amino acids 21 to 175 (155 residues), 104.7 bits, see alignment E=1.2e-33 PF12399: BCA_ABC_TP_C" amino acids 224 to 246 (23 residues), 30.9 bits, see alignment (E = 3.2e-11)

Best Hits

Swiss-Prot: 40% identical to LIVG_ECOLI: High-affinity branched-chain amino acid transport ATP-binding protein LivG (livG) from Escherichia coli (strain K12)

KEGG orthology group: K01995, branched-chain amino acid transport system ATP-binding protein (inferred from 100% identity to atu:Atu4516)

MetaCyc: 40% identical to branched chain amino acid/phenylalanine ABC transporter ATP binding subunit LivG (Escherichia coli K-12 substr. MG1655)
ABC-15-RXN [EC: 7.4.2.2]; 7.4.2.2 [EC: 7.4.2.2]; 7.4.2.2 [EC: 7.4.2.2]; 7.4.2.2 [EC: 7.4.2.2]

Predicted SEED Role

"Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1)" in subsystem ABC transporter branched-chain amino acid (TC 3.A.1.4.1) (TC 3.A.1.4.1)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.4.2.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A9CGS2 at UniProt or InterPro

Protein Sequence (254 amino acids)

>Atu4516 branched chain amino acid ABC transporter ATPase (Agrobacterium fabrum C58)
MSAIFEVSALQKNFGGLVVTNNVSLSLSPGDRTVLIGPNGAGKTTFVNLVTGNIKPSAGT
VRIAGEDVTSLSASQRVKRGLVRSFQVTRLFQDMTPEEHVALAILQREGKTGRIFGRYRA
MPSVMSEARDILGTLGLLALAETRVREIAYGQQRLIEIALAMALRPKILLLDEPAAGVPS
TETARIEQALDRLPADLAILMIEHDMDLVFRFARRVVVLAAGAVIFDGSPEDVTSNAEVR
QAYLGSYANARSAA