Protein Info for Atu4485 in Agrobacterium fabrum C58

Annotation: beta-glucosidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00232: Glyco_hydro_1
  • amino acids 9 to 447 (439 residues), 620.3 bits, E=8.1e-191
• TIGR03356: beta-galactosidase
  • amino acids 11 to 442 (432 residues), 620.6 bits, E=6.9e-191

Best Hits

Swiss-Prot: 96% identical to BGLS_AGRSA: Beta-glucosidase (abg) from Agrobacterium sp. (strain ATCC 21400)

KEGG orthology group: K05350, beta-glucosidase [EC: 3.2.1.21] (inferred from 100% identity to atu:Atu4485)

Predicted SEED Role

"Beta-galactosidase (EC 3.2.1.23)" in subsystem Galactosylceramide and Sulfatide metabolism or Lactose and Galactose Uptake and Utilization or Lactose utilization (EC 3.2.1.23)

MetaCyc Pathways

• lactose degradation III (1/1 steps found)
• cellulose degradation II (fungi) (2/3 steps found)
• neolinustatin bioactivation (2/3 steps found)
• linamarin degradation (1/2 steps found)
• lotaustralin degradation (1/2 steps found)
• linustatin bioactivation (2/4 steps found)
• coumarin biosynthesis (via 2-coumarate) (2/5 steps found)
• xyloglucan degradation II (exoglucanase) (3/8 steps found)
• α-tomatine degradation (1/6 steps found)
• firefly bioluminescence (2/14 steps found)

KEGG Metabolic Maps

• Cyanoamino acid metabolism
• Galactose metabolism
• Glycosaminoglycan degradation
• Glycosphingolipid biosynthesis - ganglio series
• Other glycan degradation
• Phenylpropanoid biosynthesis
• Sphingolipid metabolism
• Starch and sucrose metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.21 or 3.2.1.23

Sequence Analysis Tools

See Q7CV27 at UniProt or InterPro

Protein Sequence (459 amino acids)

>Atu4485 beta-glucosidase (Agrobacterium fabrum C58)
MTDPQTLAARFPGDFLFGVATASFQIEGATKADGRKPSIWDAFCNMPGHVFGRHNGDIAC
DHYNRWEEDLDLIKEMGVEAYRFSIAWPRIIPDGFGPINEKGLDFYDRLVDGCKARGIKT
YATLYHWDLPLTLMGDGGWASRSTAHAFQRYAKTVMARLGDRLDAVATFNEPWCAVWLSH
LYGIHAPGERNMEAALAAMHHINLAHGFGVEASRHVAPKVPVGLVLNAHSVIPASNSEAD
LKAAERAFQFHNGAFFDPVFKGEYPAEMMEALGDRMPVVEAEDLAIISQKLDWWGLNYYT
PMRVADDATPGAEFPATTPAPAVSEVKTDIGWEVYAPALKSLVETLYKRYDLPECYITEN
GACYNMGIENGEVNDQPRLDYYAEHLGIVADLIRDGYPMRGYFAWSLMDNFEWAEGYRMR
FGLVHVDYDTQVRTLKNSGKWYSALASRFPKGNHGVVKG