Protein Info for Atu4388 in Agrobacterium fabrum C58

Annotation: nitric oxide reductase, cytochrome b subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 448 transmembrane" amino acids 12 to 36 (25 residues), see Phobius details amino acids 56 to 74 (19 residues), see Phobius details amino acids 86 to 108 (23 residues), see Phobius details amino acids 120 to 139 (20 residues), see Phobius details amino acids 149 to 169 (21 residues), see Phobius details amino acids 181 to 206 (26 residues), see Phobius details amino acids 219 to 241 (23 residues), see Phobius details amino acids 248 to 274 (27 residues), see Phobius details amino acids 286 to 307 (22 residues), see Phobius details amino acids 327 to 347 (21 residues), see Phobius details amino acids 359 to 386 (28 residues), see Phobius details amino acids 406 to 430 (25 residues), see Phobius details PF00115: COX1" amino acids 7 to 416 (410 residues), 248.8 bits, see alignment E=5.3e-78

Best Hits

KEGG orthology group: K04561, nitric oxide reductase, cytochrome b-containing subunit I [EC: 1.7.2.5] (inferred from 100% identity to atu:Atu4388)

MetaCyc: 64% identical to nitric oxide reductase catalytic subunit (Nitrosomonas europaea)
NITRIC-OXIDE-REDUCTASE-RXN [EC: 1.7.2.5]

Predicted SEED Role

"Nitric-oxide reductase subunit B (EC 1.7.99.7)" in subsystem Denitrification (EC 1.7.99.7)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.99.7

Use Curated BLAST to search for 1.7.2.5 or 1.7.99.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A9CGJ8 at UniProt or InterPro

Protein Sequence (448 amino acids)

>Atu4388 nitric oxide reductase, cytochrome b subunit (Agrobacterium fabrum C58)
MKYQTQKVAMLYFYGAIGLFVAQILFGVVAATIYVLPNTLSELLPFNIVRMIHTNALVVW
LLMGFMGSTFYLLPEETETELYSPKLAIAQFWIFLVAAAVAVVGYMFRIHEGREFLEQPF
AIKVGIVLVVLMFLFNITMTALKGRKTTVINILLFGLWGLAIFFLFAFYNPINLALDKMY
WWYVIHLWVEGVWELIMASILAFLMIKLNGIDREVVEKWLYVIVGLALFSGILGTGHHYY
WIGAPGYWQWIGSLFSTLEVAPFFTMVIFTFVMTWKAGRKHENRAALLWSIGCSVMAFFG
AGVWGFLHTLSSVNYYTHGTQLTAAHGHLAFFGAYVMLNLAMMAYAIPEMKKRAPYNQWL
SMTSFWVMCTAMSVMTFALTFAGVVQIQLQRVMGESFMDVQDQLAIFYWIRLGSGFAVLI
SAIMFIWAVLVPGKQKQGDVARLVQPAE