Protein Info for Atu4283 in Agrobacterium fabrum C58

Annotation: ABC transporter permease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 254 PF00005: ABC_tran" amino acids 28 to 178 (151 residues), 129.6 bits, see alignment E=1.3e-41

Best Hits

Swiss-Prot: 60% identical to GLNQ_BACSU: Glutamine transport ATP-binding protein GlnQ (glnQ) from Bacillus subtilis (strain 168)

KEGG orthology group: K10041, putative glutamine transport system ATP-binding protein [EC: 3.6.3.-] (inferred from 100% identity to atu:Atu4283)

MetaCyc: 59% identical to glutamate/aspartate ABC transporter ATP binding subunit (Escherichia coli K-12 substr. MG1655)
ABC-13-RXN [EC: 7.4.2.1]; 7.4.2.1 [EC: 7.4.2.1]

Predicted SEED Role

"PROBABLE AMINO-ACID ATP-BINDING ABC TRANSPORTER PROTEIN"

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.-

Use Curated BLAST to search for 3.6.3.- or 7.4.2.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A9CGD3 at UniProt or InterPro

Protein Sequence (254 amino acids)

>Atu4283 ABC transporter permease (Agrobacterium fabrum C58)
MTTTASVTPQQTIRISNVCKSYGDYQVLKNIDAEVARGEVVVICGPSGSGKSTLIRTVNR
LEEINSGSITLDGRDIHAATRTRELNLLRSRIGFVFQSFNLFPHLSVVENVTISPIRVKG
VSPDIAQQKALALLERVGLAEKAGAYPGQLSGGQQQRVAIARALAMEPPVMLFDEPTSAL
DPEMVGEVLAVMKGLAAEGMTMLCVTHEMGFAREVADRIWFIDAGEILERATPEEFFKTP
RHPRAQRFLADLRH