Protein Info for Atu4222 in Agrobacterium fabrum C58

Annotation: sarcosine oxidase alpha subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 987 TIGR01372: sarcosine oxidase, alpha subunit family" amino acids 3 to 987 (985 residues), 1580.8 bits, see alignment E=0 PF13510: Fer2_4" amino acids 16 to 100 (85 residues), 89.8 bits, see alignment E=3.3e-29 PF12831: FAD_oxidored" amino acids 170 to 210 (41 residues), 29.4 bits, see alignment (E = 1.8e-10) PF07992: Pyr_redox_2" amino acids 170 to 304 (135 residues), 35.6 bits, see alignment E=2.5e-12 PF13450: NAD_binding_8" amino acids 173 to 230 (58 residues), 26.5 bits, see alignment 2.3e-09 PF17806: SO_alpha_A3" amino acids 502 to 583 (82 residues), 96.9 bits, see alignment E=2.5e-31 PF01571: GCV_T" amino acids 600 to 865 (266 residues), 217.4 bits, see alignment E=7.4e-68 PF08669: GCV_T_C" amino acids 894 to 979 (86 residues), 43.9 bits, see alignment E=6.2e-15

Best Hits

Swiss-Prot: 78% identical to SOXA_RHIME: Sarcosine oxidase subunit alpha (soxA) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K00302, sarcosine oxidase, subunit alpha [EC: 1.5.3.1] (inferred from 100% identity to atu:Atu4222)

Predicted SEED Role

"Sarcosine oxidase alpha subunit (EC 1.5.3.1)" in subsystem Choline and Betaine Uptake and Betaine Biosynthesis (EC 1.5.3.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.5.3.1

Use Curated BLAST to search for 1.5.3.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A9CG88 at UniProt or InterPro

Protein Sequence (987 amino acids)

>Atu4222 sarcosine oxidase alpha subunit (Agrobacterium fabrum C58)
MSSHRLKTGGQIDRSKPLSFTFDGKTMSGFAGDTLASALLANGQQLVGRSFKYHRPRGIL
TAGAAEPNALMTIGSGGRTEPNTRATMQELYAGLEAKSQNRWPSLDFDIGAMTGLLSPFL
GAGFYYKTFMWPAAFWEKIYEPFIRKAAGLGKASYEADPDAYDKCWAHCDLLVIGAGATG
LAAALAAGRAGARVIIVDEHSVAGGGLLSETATVGGRSAADFAASCIAELEALPDVTVLT
RTTAFGWYDGNVFGAVERVQKHLANPKSHVPVERLWRIVAKRALLATGAEERPLVFGGND
IPGVMMAGAMRSYLNRYAVSPGKTAAIFTTNDSGYALARDLEAQGIPLAAIIDSREQGHP
GYDGKARVIKGGVVSNAKGGKALSAIEIYANGRTENLNVDALAMSGGFSPVIHLACHRGG
KPVWSEDHAAFLAPGNLKGLELAGAVSATNGIAACLEDGGRKGAALAQNLGFAATAPAFG
MVENDIVAPPAKALWSIPGIKAKAFIDYQNDVHRKDLGLAIREGYGHVELAKRYTTNGMA
TDQGKLSNVNAVGLLAEARGVSPAEVGTTTFRPFYTPVSFGALTGAYHGHHFQPARKSPL
HGWAEKNGAVFVETGLWYRSSWFPRKGESGWRESVDREVLNVRKNAGLCDVSMLGKIEIC
GKDAAEFLNRVYCNAFLKLPVGKARYGLMLREDGMIYDDGTTSRLEENRFFMTTTTAYAA
GVMNHLEFCAQALWPDLDVRLASVTDQWAQMAIAGPKARDILQRIVDDDISDEAFPFLAA
KEVSLFGGQLHGRLFRISFSGELAYELAVPAGYGESVADALMEAGAPEGIMPYGVEALGV
LRIEKGHVTHNEINGTVVPADLGFGKMVSTTKADFIGRRMLEREGLSAANRPGLVGVMPL
DPGHSFRSGSHILARDAAATLENDQGYVTSSAFSPHLDSTIGLALVKNGAARHGEEVMVW
NGLRNEFTAARLCHPVFFDPENEKLHV