Protein Info for Atu4175 in Agrobacterium fabrum C58

Annotation: protoporphyrinogen oxidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 289 PF17827: PrmC_N" amino acids 15 to 81 (67 residues), 70.2 bits, see alignment E=7e-23 TIGR00536: methyltransferase, HemK family" amino acids 17 to 286 (270 residues), 204.7 bits, see alignment E=1.6e-64 TIGR03534: protein-(glutamine-N5) methyltransferase, release factor-specific" amino acids 31 to 285 (255 residues), 273.4 bits, see alignment E=1.8e-85 PF05175: MTS" amino acids 110 to 200 (91 residues), 58.3 bits, see alignment E=3.2e-19 PF06325: PrmA" amino acids 120 to 212 (93 residues), 35.5 bits, see alignment E=3.2e-12 PF13847: Methyltransf_31" amino acids 123 to 196 (74 residues), 47.6 bits, see alignment E=5.8e-16 PF13649: Methyltransf_25" amino acids 126 to 196 (71 residues), 41.3 bits, see alignment E=8.2e-14 PF08241: Methyltransf_11" amino acids 127 to 196 (70 residues), 23.9 bits, see alignment E=2.1e-08

Best Hits

Swiss-Prot: 100% identical to PRMC_AGRFC: Release factor glutamine methyltransferase (prmC) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K02493, methyltransferase [EC: 2.1.1.-] (inferred from 100% identity to atu:Atu4175)

Predicted SEED Role

"Protein-N(5)-glutamine methyltransferase PrmC, methylates polypeptide chain release factors RF1 and RF2"

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.-

Use Curated BLAST to search for 2.1.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A9CG70 at UniProt or InterPro

Protein Sequence (289 amino acids)

>Atu4175 protoporphyrinogen oxidase (Agrobacterium fabrum C58)
MSGGGDATVSAELIAARKRLQAAGVADPLVDARLLIADVTGFSLTDFVMKPEHPVTQDES
ARIAAMIERRAEGEPVHRILGHREFHGLDLLLSKETLEPRPDTEVLVDTLLPALKEAVSR
KGSARILDLGTGTGAICLALLKECAQASGIGSDISADALETAAKNAARNGLDSRFETIRS
NWFEKISGRFDIIVSNPPYIRTDIVATLDPEVRNHDPMAALDGGQDGLAPYRLIAADAGR
FLVENGTVGVEIGFDQRLDVSAIFASHGFSLLDAVKDYGGNDRVLTFRR