Protein Info for Atu4157 in Agrobacterium fabrum C58

Annotation: bifunctional proline dehydrogenase/pyrroline-5-carboxylate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF18327: PRODH
  • amino acids 31 to 78 (48 residues), 59.4 bits, see alignment (E = 6.6e-20)
• PF14850: Pro_dh-DNA_bdg
  • amino acids 86 to 197 (112 residues), 153.9 bits, E=4.3e-49
• PF01619: Pro_dh
  • amino acids 206 to 504 (299 residues), 350.4 bits, E=1.7e-108
• TIGR01238: delta-1-pyrroline-5-carboxylate dehydrogenase
  • amino acids 541 to 1029 (489 residues), 707.4 bits, E=4.4e-217
• PF00171: Aldedh
  • amino acids 586 to 1029 (444 residues), 339.5 bits, E=5.4e-105

Best Hits

KEGG orthology group: K13821, proline dehydrogenase / delta 1-pyrroline-5-carboxylate dehydrogenase [EC: 1.5.1.12 1.5.99.8] (inferred from 65% identity to aav:Aave_3321)

MetaCyc: 64% identical to proline dehydrogenase/1-pyrroline-5-carboxylate dehydrogenase (Pseudomonas putida KT2440)
RXN-14903 [EC: 1.5.5.2]; 1.5.5.2 [EC: 1.5.5.2]; RXN-14116 [EC: 1.5.5.2, 1.2.1.88]

Predicted SEED Role

"Proline dehydrogenase (EC 1.5.99.8) (Proline oxidase) / Delta-1-pyrroline-5-carboxylate dehydrogenase (EC 1.2.1.88)" in subsystem Proline, 4-hydroxyproline uptake and utilization or Respiratory dehydrogenases 1 (EC 1.2.1.88, EC 1.5.99.8)

MetaCyc Pathways

• L-citrulline biosynthesis (7/8 steps found)
• L-proline degradation I (3/3 steps found)
• proline to cytochrome bo oxidase electron transfer (2/2 steps found)
• superpathway of L-citrulline metabolism (9/12 steps found)
• L-N^δ-acetylornithine biosynthesis (5/7 steps found)
• L-arginine degradation I (arginase pathway) (2/3 steps found)
• ethene biosynthesis II (microbes) (1/4 steps found)
• (5R)-carbapenem carboxylate biosynthesis (1/6 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Glutamate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.1.88 or 1.5.1.12 or 1.5.5.2 or 1.5.99.8

Sequence Analysis Tools

See A9CG59 at UniProt or InterPro

Protein Sequence (1228 amino acids)

>Atu4157 bifunctional proline dehydrogenase/pyrroline-5-carboxylate dehydrogenase (Agrobacterium fabrum C58)
MADGASNAGVTIQQVGNSIFQNFAPPVREQSLLRKAVTAAYRRAEEECMAPLIEAATVTA
DQAKAIRDTARKLIEALRAKTKGTGVEGLVQEYSLSSHEGVALMCLAEALLRIPDTATRD
ALIRDKIARGDWKSHIGGGRSLFVNAATWGLVITGKLTSTVNDSGLSAALTKLIARAGEP
VIRRGVDMAMRMMGEQFVTGETIGEAIKRSKPLEEQGFQYSYDMLGEAATTAKDAERYYK
DYENAIHAIGKASAGRGIYGGPGISIKLSALHPRYARAQAERVMAELLPRVKSLMLLSKT
YDIGLNIDAEEADRLELSLDLLEELALDKDLAGWNGLGFVVQAYGRRCPFVLDYIIDLAR
RSGRRIMVRLVKGAYWDAEIKRAQVEGLEDFPVFTRKVHTDVSYIACARKLLDARDLVFP
QFATHNAQSMATIYHLAGPDFKLGDYEFQCLHGMGEPLYSEVVGKKKLDRPCRFYAPVGT
HETLLAYLVRRLLENGANSSFVNRIADPAVPVDSLLEDPVAVVKAYAVPGAQHDRIAAPA
DLFGPERKNSAGVDLSNETTLSALDKTLKAGAATEWKAAAPQAGGQTRPVLNPGDHSDIV
GHVTEPTEADVEAAMQRAAASSWPSMPVEDRAACLERAADAMQAEMPTLLGLIMREAGKS
MPNAIAEVREAIDFLRYYAAEARRNFKSDEKSLGPVVCISPWNFPLAIFIGQVAAALVAG
NPVLAKPAEETPLIAAQGVRLLHEAGVPQDAVQLLPGDGKTGAALVGSPLTAGVMFTGST
EVARLIQGQLAGRVLANGQPVPLIAETGGQNAMIVDSSALAEQVVADVIASAFDSAGQRC
SALRILCLQEDVADRTLTMLKGALHELRIGRTDQLSVDVGPVITAEAKGIIEKHIDGMRS
LGHRIEQIALAGETGKGTFVPPTIIEMKSLADLKREVFGPVLHVIRFKRDHLDRLIDEIN
ATGYGLTFGLHTRLDDTIQHVLSRVAAGNLYVNRNIIGAVVGVQPFGGRGLSGTGPKAGG
PLYLGRMTEKAPKIDRIASQQDQAAVDLARWLDENGQTVAAEAARQAAALSGLGFETELA
GPVGERNVYALHPRGKILLVPATEQGLYRQLAAALSTGNSVVIDNASGLEKAIYGLPASV
TSRIVWADDWEKSAPFAGALVEGDAERVVAINRKIAALPGPLVLFQAATTDALSGESQPY
TLDWLVEEVSVSVNTTAAGGNASLMSIG