Protein Info for Atu4092 in Agrobacterium fabrum C58
Annotation: cytochrome d oxidase subunit II
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 51% identical to CYOB_HAEIN: Probable cytochrome oxidase subunit 2 (HI_1075) from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
KEGG orthology group: K00426, cytochrome bd-I oxidase subunit II [EC: 1.10.3.-] (inferred from 100% identity to atu:Atu4092)MetaCyc: 50% identical to cytochrome bd-I subunit 2 (Escherichia coli K-12 substr. MG1655)
RXN0-5266 [EC: 7.1.1.7]; 1.11.1.- [EC: 7.1.1.7]; 1.11.1.- [EC: 7.1.1.7]
Predicted SEED Role
"Cytochrome d ubiquinol oxidase subunit II (EC 1.10.3.-)" in subsystem Bacterial RNA-metabolizing Zn-dependent hydrolases or Conserved gene cluster associated with Met-tRNA formyltransferase or Terminal cytochrome d ubiquinol oxidases or Terminal cytochrome oxidases (EC 1.10.3.-)
MetaCyc Pathways
- NADH to cytochrome bd oxidase electron transfer I (2/2 steps found)
- succinate to cytochrome bd oxidase electron transfer (2/2 steps found)
- NADH to cytochrome bd oxidase electron transfer II (1/2 steps found)
- pyruvate to cytochrome bd oxidase electron transfer (1/2 steps found)
Isozymes
Compare fitness of predicted isozymes for: 1.10.3.-
Use Curated BLAST to search for 1.10.3.- or 7.1.1.7
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A9CG21 at UniProt or InterPro
Protein Sequence (385 amino acids)
>Atu4092 cytochrome d oxidase subunit II (Agrobacterium fabrum C58) MILHQLIDYEILRVIWWLLLGVLLIGFAVTGGFDLGTGALLPFVAKTDIERRVVINSIGP VWEGNQVWLILGGGAIFAAWPPLYAVSFSGFYLAMFATLFALILRPVGFKYRSKRESPAW RSGWDWALFVGGFVPALIFGVAIGNVLQGVPFHFNDDLRIFYDGTTLFELLNPYALLCGL VSVSMLVMHGAAWLVLKTEGPVAARARSFGSIAALATTVLFALGGVFLWLGVDGYRFTSE VVTDGPSNPLSKTVEVAGGVWFANYAAHPWMMLAPALGLVFPLFAFILFRARREVLALLS SSLAIFGIIATVGLTMFPFILPSSVDPRSSLTVWDASSSHMTLFNMLVVTLIFVPMIVAY TSWVYRVLWGKVDEKAIRDDSGHAY