Protein Info for Atu4073 in Agrobacterium fabrum C58

Annotation: glycogen debranching protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 651 TIGR02100: glycogen debranching enzyme GlgX" amino acids 6 to 628 (623 residues), 886 bits, see alignment E=7.3e-271 PF02922: CBM_48" amino acids 10 to 96 (87 residues), 45.8 bits, see alignment E=6.4e-16 PF00128: Alpha-amylase" amino acids 176 to 257 (82 residues), 28.2 bits, see alignment E=1.2e-10

Best Hits

KEGG orthology group: K02438, glycogen operon protein GlgX [EC: 3.2.1.-] (inferred from 100% identity to atu:Atu4073)

Predicted SEED Role

"Glycogen debranching enzyme (EC 3.2.1.-)" in subsystem Glycogen metabolism or Trehalose Biosynthesis (EC 3.2.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.-

Use Curated BLAST to search for 3.2.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A9CG12 at UniProt or InterPro

Protein Sequence (651 amino acids)

>Atu4073 glycogen debranching protein (Agrobacterium fabrum C58)
MQIPPVFPKGATRTENGVEFTVYSRHASQIDLCLFDDAGEKETARLPMMRGEDDVHRLTH
ENAGPGTRYGFRAHGIYAPEHGLWFDPAKLLLDPYATEIDRPFRHDPSLYAFGAETGAIM
PKAILSAHDPVKRQPPRFAEGGLIYELAVKSFTQLHPYVPEDIRGTVAALAHPAILAHLK
KIGVDAVELMPITAWIDERHLPPLGLSNAWGYNPVGFIALDPRLCPGGVRELRDTVAALH
AEGIGVILDLVFNHTGESDIEGSILSLRGLDNLTAFRHPQGKPGVLVNDTGTGNTVACDH
PYIRQLIVDSLRHFVLNAGVDGFRFDLAPVLGRTAAGFDMASETLAAMHSDPVLYDRLLI
AEPWDIGPGGYQLGNFPESFLEWNDRARDDMRRFWRGDAGTTGALANALSGSSPIFSRHG
RLKSRSVNFLAAHDGFTLFDLVSHERKHNEKNGENNRDGHNDNHSWNNGFEGLTDDPAIV
AARLADVKALLSTLFVSRGTLMLTAGDEGGRSQQGNNNAYCQDNEISWVDWSSLVPELID
HTAFLAALRKRFGVFSESGFFSGRGDVTWLAPGGEPMTVADWERPDGQAFAMVLSTPDRE
TGKATELAVLINRGRDIVPFTLPGDGWRSIGTDFGNPAFLPARSVVFYLRG