Protein Info for Atu3914 in Agrobacterium fabrum C58

Annotation: alpha-amylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 495 PF00128: Alpha-amylase" amino acids 24 to 368 (345 residues), 87.1 bits, see alignment E=1.8e-28

Best Hits

Swiss-Prot: 63% identical to AMY2_ECOLI: Cytoplasmic alpha-amylase (amyA) from Escherichia coli (strain K12)

KEGG orthology group: K01176, alpha-amylase [EC: 3.2.1.1] (inferred from 100% identity to atu:Atu3914)

MetaCyc: 63% identical to alpha-amylase (Escherichia coli K-12 substr. MG1655)
Alpha-amylase. [EC: 3.2.1.1]

Predicted SEED Role

"Cytoplasmic alpha-amylase (EC 3.2.1.1)" in subsystem Maltose and Maltodextrin Utilization (EC 3.2.1.1)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q7CTL9 at UniProt or InterPro

Protein Sequence (495 amino acids)

>Atu3914 alpha-amylase (Agrobacterium fabrum C58)
MAGRTLLQFFHWYYPDGGKLWSEVAEKAESLAKMGITDVWLPPAYKGAAGGYSVGYDTYD
LFDLGEFDQKGTVATKYGDRAALEHAGKTLKDNGIRVIHDVVLNHKMGADEKEKVRVRRV
NPDDRTDIDDEDFPALAYTRFTFPGRNGKHSKFIWDLKCFSGVDHIEEPTEDGIFRLVNE
YGDGEWNEEVDQENGNFDYLMGADVEFRNRAVYEELKYWGRWLSEQVQVDGFRLDAAKHI
PAWFFRDWVGHMRETVDPDLFVVAEYWHPDLEALKSYLELVDKQLMLFDVALHHSFHDAS
KQGGDFDMRSIFDGSLVSAVPDHAVTLVDNHDTQPLQSLEAPVEPWFKPLAYAIILLREE
GVPCVFYPDLFGTSYTDTGNDGNEYKIDIPAIECLPKLIEARSRFANGPQTDIFDDASCI
AFIRHGTADAPGCVVVMSNGEPGEKQADLGPERAGSVWRDFLGHREEHITLDESGKGTFP
TNGGSVSVWVPADSE