Protein Info for Atu3913 in Agrobacterium fabrum C58

Annotation: biotin carboxylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 576 PF00289: Biotin_carb_N" amino acids 1 to 109 (109 residues), 150.3 bits, see alignment E=9.7e-48 PF02786: CPSase_L_D2" amino acids 114 to 321 (208 residues), 256.2 bits, see alignment E=8e-80 PF02222: ATP-grasp" amino acids 137 to 291 (155 residues), 34.9 bits, see alignment E=4.1e-12 PF07478: Dala_Dala_lig_C" amino acids 143 to 290 (148 residues), 29.3 bits, see alignment E=2.1e-10 PF02785: Biotin_carb_C" amino acids 334 to 443 (110 residues), 112.4 bits, see alignment E=4.1e-36 PF00364: Biotin_lipoyl" amino acids 509 to 574 (66 residues), 53 bits, see alignment E=8.9e-18 PF13533: Biotin_lipoyl_2" amino acids 510 to 547 (38 residues), 26.4 bits, see alignment 1.6e-09

Best Hits

KEGG orthology group: K11263, acetyl-/propionyl-CoA carboxylase, biotin carboxylase, biotin carboxyl carrier protein [EC: 6.3.4.14] (inferred from 100% identity to atu:Atu3913)

Predicted SEED Role

"Urea carboxylase (EC 6.3.4.6) without Allophanate hydrolase 2 domains" (EC 6.3.4.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.3.4.14

Use Curated BLAST to search for 6.3.4.14 or 6.3.4.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A9CFU1 at UniProt or InterPro

Protein Sequence (576 amino acids)

>Atu3913 biotin carboxylase (Agrobacterium fabrum C58)
MKKVLIANRGEIAVRIIRACRDYGLQSVAVYADPDQDALFVRLADEAYALEGVRPAETYL
DISKLIAIAKRAGADAVHPGYGFLSERAEFAQAVIDAGLIWIGPDPDVIEALGDKVMARR
IATGVGAPLVAGSDGPVSSAAEVTAFAEQHGLPVAIKAAHGGGGRGLKVAWKMEEIAELY
ESAVREATVAFGRGECFLERFLDRPRHIEAQVIADKHGNVLVLGTRDCSLQRRNQKLIEE
APAPFLSDEQRQKIHDAAKAICAAAGYSGAGTVEFLLGVDGTISFLEVNTRLQVEHPVTE
ETTGIDLVIEQFRIAEGHKLRVLETPEPRGHSMEFRINAEDPGRGFLPTPGLISVFDAPS
GPGIRMDSGVISGSSIPGVFDSLMAKLIVTGVDRDQVLRRARRALREFRIEGIATVLPFH
RAAIETEDFIGTDGFKVHTRWIETDFAAMPDAMERPAPADDPSITRTYLEIDGKRVSVGL
PSILLSSLGTVSGGNASAPSAAVEKKEGEMTAPVSGTLQSFKVKDGETVSEGDLLAVMEA
MKMETQIVATRAGKVRLIVKEGDYLQAGATLIDIAG