Protein Info for Atu3899 in Agrobacterium fabrum C58

Annotation: siroheme synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 475 TIGR01470: siroheme synthase, N-terminal domain" amino acids 7 to 213 (207 residues), 281.6 bits, see alignment E=3.5e-88 PF13241: NAD_binding_7" amino acids 9 to 121 (113 residues), 63.8 bits, see alignment E=4.1e-21 PF14824: Sirohm_synth_M" amino acids 130 to 150 (21 residues), 25.6 bits, see alignment (E = 1.4e-09) PF10414: CysG_dimeriser" amino acids 157 to 204 (48 residues), 37.8 bits, see alignment 2.6e-13 TIGR01469: uroporphyrinogen-III C-methyltransferase" amino acids 221 to 456 (236 residues), 273.2 bits, see alignment E=2.2e-85 PF00590: TP_methylase" amino acids 223 to 434 (212 residues), 163.2 bits, see alignment E=1.8e-51

Best Hits

KEGG orthology group: K02302, uroporphyrin-III C-methyltransferase / precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase [EC: 1.3.1.76 2.1.1.107 4.99.1.4] (inferred from 100% identity to atu:Atu3899)

Predicted SEED Role

"Siroheme synthase / Precorrin-2 oxidase (EC 1.3.1.76) / Sirohydrochlorin ferrochelatase (EC 4.99.1.4) / Uroporphyrinogen-III methyltransferase (EC 2.1.1.107)" in subsystem Experimental tye or Heme and Siroheme Biosynthesis or Coenzyme B12 biosynthesis or Dissimilatory nitrite reductase (EC 1.3.1.76, EC 2.1.1.107, EC 4.99.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.1.76, 2.1.1.107, 4.99.1.4

Use Curated BLAST to search for 1.3.1.76 or 2.1.1.107 or 4.99.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q7CTK5 at UniProt or InterPro

Protein Sequence (475 amino acids)

>Atu3899 siroheme synthase (Agrobacterium fabrum C58)
MERLAKLPVFWGLEGKRVVLTGGSDGAAWKAELLLACGAQLDLYCEESGLSESLATLVAK
SPMLTWHDRCWDADIFKGAELALADCEAEEEAGRFYHAARAAGVPVNVIDKPEFCQFQFG
SIVNRSPVVVSISTDGAAPILAQAIRRRIETLLPLSLKDWGALAQTIRERVNLRLAPGAA
RRSFWEKFVDRAFTERLDEGSEERLLKDVATRTGLAESGRGFVTLVGAGPGDAELLTLKA
VRALQAADVILFDDLVSAEVLELARREAKRMLVGKRGGRESCKQEDINDMMIRFAKAGRR
VVRLKSGDPMIFGRAGEEIAALEAENIPVEVVPGITAASAMASRLGVSLTHRDHAQSVRF
VTGHSRQGKLPENIDWQSLSNPSVTTVFYMGGRTAADIQSCLLAHGMPASTPVVVMISVS
RVNEQRWCGSLAQLVAAVERLGVNEPVLIGVGDAFRSASVNGGEQTAAAPFQKAG