Protein Info for Atu3783 in Agrobacterium fabrum C58

Annotation: glutamate synthase subunit beta

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01317: glutamate synthase, NADH/NADPH, small subunit
  • amino acids 3 to 483 (481 residues), 597.8 bits, E=8.5e-184
• PF14691: Fer4_20
  • amino acids 24 to 131 (108 residues), 91.2 bits, E=1.6e-29
• PF07992: Pyr_redox_2
  • amino acids 146 to 465 (320 residues), 68.9 bits, E=2.1e-22
• PF03486: HI0933_like
  • amino acids 147 to 181 (35 residues), 24.1 bits, 5.9e-09
• PF01494: FAD_binding_3
  • amino acids 147 to 180 (34 residues), 23.4 bits, see alignment (E = 1.3e-08)
• PF13450: NAD_binding_8
  • amino acids 150 to 184 (35 residues), 33.5 bits, 1.7e-11
• PF01593: Amino_oxidase
  • amino acids 156 to 185 (30 residues), 22.9 bits, see alignment (E = 1.9e-08)

Best Hits

KEGG orthology group: K00266, glutamate synthase (NADPH/NADH) small chain [EC: 1.4.1.13 1.4.1.14] (inferred from 100% identity to atu:Atu3783)

Predicted SEED Role

"Glutamate synthase [NADPH] small chain (EC 1.4.1.13)" in subsystem Ammonia assimilation or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 1.4.1.13)

MetaCyc Pathways

• L-citrulline biosynthesis (7/8 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (4/4 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• ammonia assimilation cycle I (2/2 steps found)
• superpathway of L-citrulline metabolism (9/12 steps found)
• L-glutamate biosynthesis IV (1/1 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-glutamine degradation II (1/1 steps found)
• superpathway of ammonia assimilation (plants) (2/3 steps found)
• L-glutamate and L-glutamine biosynthesis (4/7 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Glutamate metabolism
• Nitrogen metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.4.1.13, 1.4.1.14

Use Curated BLAST to search for 1.4.1.13 or 1.4.1.14

Sequence Analysis Tools

See A9CFN1 at UniProt or InterPro

Protein Sequence (484 amino acids)

>Atu3783 glutamate synthase subunit beta (Agrobacterium fabrum C58)
MGKVTGFLEIDRQVGKYQPASDRIRHFREFTIPMSDAEVQKQAARCMDCGIPYCHGPTGC
PVHNQIPDWNDLVYNNNWEEAIRNLHSTNNFPEFTGRVCPAPCEEACTLNLEDAPVAIKT
VEQAIADKAYELGFIVPKPATIHTGKKVAIIGSGPSGMAAAQQLGRAGHEVHVYERESKA
GGLLRYGIPDFKMEKNFIDRRVEQIKGEGVTFHCGVNVGVDVTVEALLADHDAVLYCGGS
ETPRPAGIGGADFNGVYDAMPYLVQQNRRVGRENIDSVGWPADPILAGGKHVVVVGGGDT
ASDCVGTAFRQGAVKVTQLDIRPMPPEKEDKLAVWPFWATKMRTSSSQAEGAIREFQVAT
LEFVGEDGVLTGVKCCQVDDRRKPIAGTEFIIKADLAFIAIGFSGPFTDSVLKEMEGKLT
LNTDRRGSTNVVANETDYRTSVDKLWTAGDVRRGQSLVVWAIREGRQAARAIDEALMGAT
VLPR