Protein Info for Atu3722 in Agrobacterium fabrum C58

Annotation: Holliday junction DNA helicase RuvB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 346 PF05496: RuvB_N" amino acids 21 to 179 (159 residues), 266.5 bits, see alignment E=2.5e-83 TIGR00635: Holliday junction DNA helicase RuvB" amino acids 25 to 327 (303 residues), 446.5 bits, see alignment E=2.1e-138 PF06068: TIP49" amino acids 26 to 85 (60 residues), 25.2 bits, see alignment E=3.3e-09 PF00004: AAA" amino acids 56 to 176 (121 residues), 79.2 bits, see alignment E=1.4e-25 PF17864: AAA_lid_4" amino acids 182 to 255 (74 residues), 98.6 bits, see alignment E=4.9e-32 PF05491: RuvB_C" amino acids 257 to 326 (70 residues), 95.4 bits, see alignment E=5.7e-31

Best Hits

Swiss-Prot: 100% identical to RUVB_AGRFC: Holliday junction ATP-dependent DNA helicase RuvB (ruvB) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K03551, holliday junction DNA helicase RuvB (inferred from 100% identity to atu:Atu3722)

MetaCyc: 61% identical to Holliday junction branch migration complex subunit RuvB (Escherichia coli K-12 substr. MG1655)
3.1.22.4-RXN [EC: 3.1.21.10]

Predicted SEED Role

"Holliday junction DNA helicase RuvB" in subsystem DNA-replication or RuvABC plus a hypothetical

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.21.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8U9K6 at UniProt or InterPro

Protein Sequence (346 amino acids)

>Atu3722 Holliday junction DNA helicase RuvB (Agrobacterium fabrum C58)
MSDADRLITPEKRGEDIDTTLRPQSLDDFTGQAEARANLKVFIEAAKNRGEALDHVLFVG
PPGLGKTTLAQIMAKELGVNFKSTSGPVIAKAGDLAALLTNLEERDVLFIDEIHRLNPAV
EEILYPAMEDFQLDLIIGEGPAARSVKIDLSKFTLVAATTRLGLLTTPLRDRFGIPVRLA
FYTVDELELIVRRGARLMGLNMTDGGAREIARRARGTPRIAGRLLRRVRDFAEVARAEAV
TREIADEALTRLLVDNMGLDQLDMRYLTMIAVNFGGGPVGIETIAAGLSEPRDAIEDIIE
PYMIQQGFIQRTPRGRILTATAWKHLGLQPPKDLEAAQFRLTLEDD