Protein Info for Atu3614 in Agrobacterium fabrum C58

Annotation: glyoxalase II

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 256 TIGR03413: hydroxyacylglutathione hydrolase" amino acids 9 to 256 (248 residues), 303 bits, see alignment E=7.3e-95 PF00753: Lactamase_B" amino acids 27 to 172 (146 residues), 73.1 bits, see alignment E=3.2e-24 PF16123: HAGH_C" amino acids 173 to 256 (84 residues), 97.5 bits, see alignment E=4.7e-32

Best Hits

Swiss-Prot: 100% identical to GLO2_AGRFC: Hydroxyacylglutathione hydrolase (gloB) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K01069, hydroxyacylglutathione hydrolase [EC: 3.1.2.6] (inferred from 100% identity to atu:Atu3614)

Predicted SEED Role

"Hydroxyacylglutathione hydrolase (EC 3.1.2.6)" in subsystem Glutathione: Non-redox reactions or Methylglyoxal Metabolism (EC 3.1.2.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.2.6

Use Curated BLAST to search for 3.1.2.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8U9W1 at UniProt or InterPro

Protein Sequence (256 amino acids)

>Atu3614 glyoxalase II (Agrobacterium fabrum C58)
MKPLEIDVFLCRSDNFGVLLHDPESGATAAIDAPEEGPILRALDGHGWKLTHIFTTHHHQ
DHVEANLVLKDKFRCEVHGPYDEAIAIPGLDRSQADGDEFEFAGRRVQVIATPGHTAGHI
CYYLPDDGLLFAADTLFAMGCGRLFERPAADMWHSFQKLMALPDDTRVYFGHEYTLSNAR
FALTVDPDNAVLRERAARVETARQANEFTIPTTIGLEKQTNPYMRVADAGIRRHLGLEGA
ADADVFAEIRTRKDNF