Protein Info for Atu3580 in Agrobacterium fabrum C58

Annotation: methylmalonyl-CoA mutase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00641: methylmalonyl-CoA mutase N-terminal domain
  • amino acids 27 to 545 (519 residues), 879 bits, E=8.5e-269
• PF01642: MM_CoA_mutase
  • amino acids 29 to 541 (513 residues), 799 bits, E=1.7e-244
• TIGR00640: methylmalonyl-CoA mutase C-terminal domain
  • amino acids 580 to 707 (128 residues), 183.6 bits, E=1.5e-58
• PF02310: B12-binding
  • amino acids 583 to 698 (116 residues), 72.7 bits, E=2.4e-24

Best Hits

Swiss-Prot: 83% identical to MUTB_RHIME: Methylmalonyl-CoA mutase (bhbA) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K01847, methylmalonyl-CoA mutase [EC: 5.4.99.2] (inferred from 100% identity to atu:Atu3580)

MetaCyc: 77% identical to methylmalonyl-CoA mutase (Cereibacter sphaeroides)
Methylmalonyl-CoA mutase. [EC: 5.4.99.2]

Predicted SEED Role

"Methylmalonyl-CoA mutase (EC 5.4.99.2)" in subsystem Propionyl-CoA to Succinyl-CoA Module or Serine-glyoxylate cycle (EC 5.4.99.2)

MetaCyc Pathways

• propanoyl CoA degradation I (3/3 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (8/10 steps found)
• superpathway of anaerobic energy metabolism (invertebrates) (13/17 steps found)
• 2-oxobutanoate degradation I (3/4 steps found)
• pyruvate fermentation to propanoate I (4/7 steps found)
• conversion of succinate to propanoate (1/3 steps found)
• 3-hydroxypropanoate cycle (8/13 steps found)
• methylaspartate cycle (12/19 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (11/18 steps found)
• superpathway of L-methionine salvage and degradation (8/16 steps found)
• L-glutamate degradation VIII (to propanoate) (4/11 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (5/13 steps found)
• superpathway of the 3-hydroxypropanoate cycle (8/18 steps found)
• crotonyl-CoA/ethylmalonyl-CoA/hydroxybutyryl-CoA cycle (engineered) (3/14 steps found)

KEGG Metabolic Maps

• Propanoate metabolism
• Valine, leucine and isoleucine degradation

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.4.99.2

Sequence Analysis Tools

See A9CFE8 at UniProt or InterPro

Protein Sequence (713 amino acids)

>Atu3580 methylmalonyl-CoA mutase (Agrobacterium fabrum C58)
MSGEKTVRDWLQLAEQELKRSPETLVWHTPEGIEVKPLYTADDLQDVSHLNSLPGFAPFT
RGPRATMYAGRPWTIRQYAGFSTAEASNAFYRKALAAGQQGVSVAFDLATHRGYDSDHPR
VEGDVGKAGVAIDSVEDMKILFDGIPLEKVSVSMTMNGAVIPVLANFIVAGEEQGVPRAA
LSGTIQNDILKEFMVRNTYIYPPEPSMRIIADIIEYTAKEMPKFNSISISGYHMQEAGAT
LVQELAFTLADGREYVRAALAKGLNVDDFAGRLSFFFAIGMNFFMEAAKLRAARLLWSRI
MEEFKPQKASSLMLRTHCQTSGVSLQEQDPYNNIVRTAFEAMSAVLGGTQSLHTNSFDEA
IALPTEFSARIARNTQLILQHETGVTKVVDPLAGSYYVESLTDELAEKAWALIEEVETLG
GMTKAVADGLPKRLIEEAATRRQAAVDKGEEVIVGVNRYRLETEEDIDVLDIDNAAVRAA
QIRRIEETRRRRDGKEATAALEALSEIARSGKGNILEAAVVASRARATVGEISDALRVAF
GDHAAVPEVVSDIYGEAYKDEPELATLSARLSGVAERIGKKPRIMVAKLGQDGHDRGAKV
IASAFGDIGFDVLAGPLFQTPAEAAELAVQNKVHVVGMSSLAAGHKTLAPQLVEALKQKG
ADDIIVVVGGVIPRQDYQFLLDHGVAAIFGPGTNVMDAANKVLDLLEGIRRNA