Protein Info for Atu3485 in Agrobacterium fabrum C58

Annotation: short chain dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 698 TIGR02632: rhamnulose-1-phosphate aldolase/alcohol dehydrogenase" amino acids 24 to 698 (675 residues), 1164.3 bits, see alignment E=0 PF00596: Aldolase_II" amino acids 33 to 219 (187 residues), 55.8 bits, see alignment E=1.4e-18 PF00106: adh_short" amino acids 438 to 621 (184 residues), 158.5 bits, see alignment E=3.8e-50 PF01370: Epimerase" amino acids 440 to 511 (72 residues), 22.5 bits, see alignment E=1.7e-08 PF08659: KR" amino acids 440 to 606 (167 residues), 43.7 bits, see alignment E=7.7e-15 PF13561: adh_short_C2" amino acids 445 to 691 (247 residues), 168.9 bits, see alignment E=3.7e-53

Best Hits

Swiss-Prot: 44% identical to YUXG_BACSU: Uncharacterized oxidoreductase YuxG (yuxG) from Bacillus subtilis (strain 168)

KEGG orthology group: None (inferred from 100% identity to atu:Atu3485)

MetaCyc: 44% identical to bifunctional L-rhamnulose 1-phosphate aldolase/lactaldehyde dehydrogenase (Bacillus subtilis subtilis 168)
Rhamnulose-1-phosphate aldolase. [EC: 4.1.2.19]; Lactaldehyde dehydrogenase. [EC: 4.1.2.19, 1.2.1.22]

Predicted SEED Role

"Predicted rhamnulose-1-phosphate aldolase (EC 4.1.2.19) / Predicted lactaldehyde dehydrogenase (EC 1.2.1.22)" in subsystem L-rhamnose utilization (EC 1.2.1.22, EC 4.1.2.19)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.22

Use Curated BLAST to search for 1.2.1.22 or 4.1.2.19

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q7CSK9 at UniProt or InterPro

Protein Sequence (698 amino acids)

>Atu3485 short chain dehydrogenase (Agrobacterium fabrum C58)
MTGTPRLLESRWDDAYAQTLDEPGKLLYRSNLLGADKRITNYGGGNTSAKVLEIDPLTGE
KVRVMWVKGSGGDVGTIKLDGFATLYLEKLEALKGIYKGVEDEDRMVGFLPHCTFNLNPR
AASIDTPLHGFVPFDHVDHMHPDAIIAIAASKNSKELTQQIFGDDIGWLPWRRPGFQLGL
DLEAFVKANPTAKGVVLESHGLFTWDDDAKACYELTLAIINKAIEWFAAETEGKTIFGGA
LAKSLPVAERRAIAARLMPEIRGRIGREERKLGHFDDQDAVLEFVNSKDLKPLGNLGTSC
PDHFLRTKIRPLILDLDTAKPDVDALTAGLDKALEAYRADYSRYYEACRHDNSPKMRDPN
PVIFLIPGVGMLSFAKDKATARIAGEFYVNAINVMRGASTVSEYQGLPEQEAFDIEYWLL
EEAKLQRMPKPKSLAGRVAFVTGGAGGIGRATAERLADEGACVVLADIDEAALEAAKGDF
VKRYSGDAVRTVKLDVTQEDDVISAFAEASVEFGGVDILVSNAGIASSAPVEDTTLAMWN
KNIDILATGYFLVSREAFRLLRRQNLGGNIVFVASKNGLASSPNASAYCTAKAAEIHLAR
CLALEGAEAGIRVNTVNPDAVLRGSKIWNGEWREQRAASSKIEVTDLEEHYRKRSMLKLN
VFPEDIAEAIYFLASDASAKSTGNIINVDAGNAQSFTR