Protein Info for Atu3479 in Agrobacterium fabrum C58

Annotation: 3-methylcrotonyl-CoA carboxylase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 663 PF00289: Biotin_carb_N" amino acids 1 to 110 (110 residues), 158.9 bits, see alignment E=2.2e-50 PF02786: CPSase_L_D2" amino acids 116 to 321 (206 residues), 258.2 bits, see alignment E=2.1e-80 PF02222: ATP-grasp" amino acids 131 to 291 (161 residues), 35.2 bits, see alignment E=4e-12 PF07478: Dala_Dala_lig_C" amino acids 139 to 290 (152 residues), 32.7 bits, see alignment E=2.3e-11 PF02785: Biotin_carb_C" amino acids 335 to 442 (108 residues), 118.5 bits, see alignment E=5.8e-38 PF00364: Biotin_lipoyl" amino acids 594 to 657 (64 residues), 53.3 bits, see alignment E=8.3e-18

Best Hits

KEGG orthology group: K01968, 3-methylcrotonyl-CoA carboxylase alpha subunit [EC: 6.4.1.4] (inferred from 100% identity to atu:Atu3479)

Predicted SEED Role

"Methylcrotonyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.4)" in subsystem HMG CoA Synthesis or Leucine Degradation and HMG-CoA Metabolism or Serine-glyoxylate cycle (EC 6.4.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.4.1.4

Use Curated BLAST to search for 6.4.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q7CSK5 at UniProt or InterPro

Protein Sequence (663 amino acids)

>Atu3479 3-methylcrotonyl-CoA carboxylase subunit alpha (Agrobacterium fabrum C58)
MFSKILIANRGEIACRIIRTAASMGIRTVAVYSDADRDAMHVALADEAIAIGPAPARASY
LDGEKIIAAAKASGAEAIHPGYGFLSENAGFAEACASAGLVFIGPPPEAIRAMGGKSEAK
ALMATAGVPIVPGYHGDEQAEKRLASEADKIGYPVLLKASAGGGGKGMRVVRQKHDFAAE
LAGAKREALAAFGNDRMLIEKYLERPRHVEVQVFADGHGNCVSLFERDCSIQRRHQKVIE
EAPAPGLPDDLRQRMYDAATAAARAIDYRGAGTIEFLLDPSGFYFMEMNTRLQVEHPVTE
YITGLDLVEWQLRVANGEALPKDWADLRINGHAIEARIYAEDPAHDFLPSIGTISHLVFP
DEGPHLRIDSGIRAGDAITVHYDPMIAKLIVWDHDRPAAVRRLKVALEKLAICGVTSNAT
FLARLAGLETFAAADLDTGFIARNEADLFAPQAVGDNEIALAALGFLLSRKNASTNPQAQ
ADPHSPWNTANAFRLNAPARETLHFTMQNRPIDLGVAHEADSFSLEMGGHTIRAYGDLSD
NGRFRATINDRTWKGYFFAESDSYALFLHGEHYRISQPDPVDIADSSGHTGGLEAPMPGV
IRAVLSQSGAAVETGDALVVMEAMKMEHTIRAPAKGIVTAVNCAEGDMVVAGAVLVDFEP
EGI