Protein Info for Atu3445 in Agrobacterium fabrum C58

Annotation: hippurate hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 399 TIGR01891: amidohydrolase" amino acids 26 to 384 (359 residues), 420.9 bits, see alignment E=2.4e-130 PF01546: Peptidase_M20" amino acids 86 to 393 (308 residues), 149.8 bits, see alignment E=1.1e-47 PF07687: M20_dimer" amino acids 195 to 293 (99 residues), 33.2 bits, see alignment E=4.3e-12

Best Hits

Swiss-Prot: 43% identical to HIPO_CAMJE: Hippurate hydrolase (hipO) from Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)

KEGG orthology group: K01451, hippurate hydrolase [EC: 3.5.1.32] (inferred from 100% identity to atu:Atu3445)

MetaCyc: 39% identical to jasmonoyl-L amino acid hydrolase ILL4 (Arabidopsis thaliana col)
3.5.1.M7 [EC: 3.5.1.M7]; 3.5.1.M7 [EC: 3.5.1.M7]; RXN-18481 [EC: 3.5.1.M7, 3.5.1.127]; 3.5.1.127 [EC: 3.5.1.M7, 3.5.1.127]

Predicted SEED Role

"Hippurate hydrolase (EC 3.5.1.32)" (EC 3.5.1.32)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.32

Use Curated BLAST to search for 3.5.1.127 or 3.5.1.32 or 3.5.1.M7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A9CF81 at UniProt or InterPro

Protein Sequence (399 amino acids)

>Atu3445 hippurate hydrolase (Agrobacterium fabrum C58)
MNIAVDPVKTPAPKVIDRIGTYVPELVSIRHDLHQHPEIGFEEVRTSGIVAEKLTSWGIE
VHRGYGKTGVVGVLRGRHAGNRSIGLRADMDALPMPEETGLPYTSVYPGKFHGCGHDVHT
TILLGTARYLAETRDFAGTVVFIFQPAEEGLGGARAMIADGLFKDFPVDEIYGLHNSTHA
APNYLKVSPGTILAGADFFDIRLKGRGAHAAHPDVSRDPVPATGELIQALQTIVSRNVSP
TDPAVLSITRIEAGSAYNVIPETASIAGTVRAFSDSVRETIRGRIRTISDHIAAAHGLTA
EVDIRDIFSVLTNDDASVEIVASVAREVLGDERVSTEPGRFMGSEDFADLLKHAPGAFFT
LGHSGTVPAHNPGFIVDDAILPVGATLFARLVETRLRPA