Protein Info for Atu3364 in Agrobacterium fabrum C58

Annotation: D-amino acid dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 417 transmembrane" amino acids 7 to 25 (19 residues), see Phobius details PF01266: DAO" amino acids 8 to 398 (391 residues), 210.9 bits, see alignment E=1e-65 PF00890: FAD_binding_2" amino acids 8 to 54 (47 residues), 24.3 bits, see alignment 4.3e-09 PF13450: NAD_binding_8" amino acids 11 to 45 (35 residues), 22 bits, see alignment 4.1e-08

Best Hits

KEGG orthology group: K00285, D-amino-acid dehydrogenase [EC: 1.4.99.1] (inferred from 100% identity to atu:Atu3364)

Predicted SEED Role

"D-amino acid dehydrogenase small subunit (EC 1.4.99.1)" in subsystem Pyruvate Alanine Serine Interconversions or Respiratory dehydrogenases 1 (EC 1.4.99.1)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.4.99.1

Use Curated BLAST to search for 1.4.99.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A9CF30 at UniProt or InterPro

Protein Sequence (417 amino acids)

>Atu3364 D-amino acid dehydrogenase (Agrobacterium fabrum C58)
MTKTLKCDVLVIGTGIIGSMAALYLQNAGRDVVLLERGEVARGASSGNAGILAFPEIIPI
PAPGIMKKAPRWLFDPLGPLSVPPAYAPKIAPWLWRFWRASAERNFRHGLKSLTEINRLA
AVEMAHVAAMPELSHLVSKTGTLDLYDSEANLNAARRDWEEKQSAGFAFERVGRNEIDAL
QPGLAPQFRHAIFSADGLQVSDPYDFTRAIFDLVVARGASLRKGEAERIDAVGESTIVTL
ENGDKIDADKVVIAGGAWSKKLAETLGNIVPLETERGYNTTLPAGAFDLTRQLYFNDHGF
VVTPLSTGIRVGGAVELGGLELKPNFRRAEAMLKKAGRFLPGLKLEGGQQWMGFRPSMPD
CLPVIGTARATPSVIYAFGHGHLGLTQSAATARLVTQLASGKETAISVDPFRPGRFS